Towards a high reliability of the PCR amplification process in DNA computing

Polymerase Chain Reaction (PCR) is the most important process in DNA computing. When the concentration of a DNA sequence is too little to investigate, PCR could amplify the sequence with a polymerase. PCR is frequently applied to obtain a result in DNA computing, because the result is generally shown by a little amount of DNA sequence. Therefore, the reliability of PCR must be assured for DNA computing. The authors define the reproducibility of PCR as the reliability. Similarly, the reaction time is defined as a reaction cost, and other parameters are defined as control factors in quality engineering. By adjusting the factors, we improve a PCR performance shown by the reproducibility and reaction cost.