{"title":"长合成寡核苷酸在基因分析中的应用:探针长度和严格条件对杂交特异性的影响","authors":"Yoshio Kajimura , Jacqueline Krull , Shoichi Miyakoshi , Keiichi Itakura , Hiroo Toyoda","doi":"10.1016/0735-0651(90)90031-A","DOIUrl":null,"url":null,"abstract":"<div><p>Two different lengths of long unique synthetic oligonucleotide probes (37- and 48-mers) specific for human major histocompatibility complex (MHC) class II beta genes were synthesized. These oligonucleotides were utilized to examine factors influencing hybridization specificity. Both probe length and stringency of washing conditions were found to be crucial factors for sequence-specific hybridization.</p></div>","PeriodicalId":77714,"journal":{"name":"Gene analysis techniques","volume":"7 4","pages":"Pages 71-79"},"PeriodicalIF":0.0000,"publicationDate":"1990-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0735-0651(90)90031-A","citationCount":"4","resultStr":"{\"title\":\"Application of long synthetic oligonucleotides for gene analysis: Effect of probe length and stringency conditions on hybridization specificity\",\"authors\":\"Yoshio Kajimura , Jacqueline Krull , Shoichi Miyakoshi , Keiichi Itakura , Hiroo Toyoda\",\"doi\":\"10.1016/0735-0651(90)90031-A\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Two different lengths of long unique synthetic oligonucleotide probes (37- and 48-mers) specific for human major histocompatibility complex (MHC) class II beta genes were synthesized. These oligonucleotides were utilized to examine factors influencing hybridization specificity. Both probe length and stringency of washing conditions were found to be crucial factors for sequence-specific hybridization.</p></div>\",\"PeriodicalId\":77714,\"journal\":{\"name\":\"Gene analysis techniques\",\"volume\":\"7 4\",\"pages\":\"Pages 71-79\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1990-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0735-0651(90)90031-A\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gene analysis techniques\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/073506519090031A\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene analysis techniques","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/073506519090031A","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Application of long synthetic oligonucleotides for gene analysis: Effect of probe length and stringency conditions on hybridization specificity
Two different lengths of long unique synthetic oligonucleotide probes (37- and 48-mers) specific for human major histocompatibility complex (MHC) class II beta genes were synthesized. These oligonucleotides were utilized to examine factors influencing hybridization specificity. Both probe length and stringency of washing conditions were found to be crucial factors for sequence-specific hybridization.
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