A high throughput surface plasmon resonance imaging electrochemical system parallel monitoring fifty spots

This research introduces a homemade high throughput surface plasmon resonance imaging electrochemical system which can detect more than 50 spots simultaneously. The SPRI system was constructed in the Kretschmann configuration and the SPR sensor chip served as the working electrode in the EC module. K3Fe(CN)6 solution were tested by the cyclic voltammetry (CV) method and the SPRI method recording more than 50 spot signals simultaneously. The multiple sensing spots were unanimous due to the ununiformity of incident light and sensor surface. In order to eliminate ununiformity of different spots, glycerol gradient solution was tested, and a calibration and normalization method was applied.