低强度激光照射及可见光和红外极化照射对大鼠肝脏线粒体的影响

T. Ovsyannikova, A. Kovalenko, I. Zabelina, Natalya Hmil, T. Mishchenko, A. Gladkih, A. Levchenko
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引用次数: 0

摘要

本文考虑了低强度激光辐照(LILI;633 nm, 20-40 mW/cm2)以及可见光和红外极化电磁辐射(VIRPEI;480-3400 nm, 20-40 mW/cm2)作用于正常和2型糖尿病大鼠肝线粒体。结果表明,糖尿病大鼠线粒体吸氧速率一方面较对照组大鼠降低,另一方面又因LILI作用而升高。对照组大鼠的呼吸数据在激光照射后无明显变化。由于VIRPEI的影响,线粒体功能下降。呼吸过程是各种生物(包括多细胞生物和单细胞生物,如细菌)接受能量的基本过程。由于生物呼吸是基于电子传递链的功能,因此分离线粒体是研究呼吸过程的模型系统。这些效应的应用领域首先是生物技术,特别是细胞和组织的培养。
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Effect of Low Intensity Laser Irradiation as well as Visible and Infra-Red Polarized Irradiation on Rat Liver Mitochondria
The paper considers the effects of low intensity laser irradiation (LILI; 633 nm, 20–40 mW/cm2) as well as visible and infra-red polarized electromagnetic irradiation (VIRPEI; 480-3400 nm, 20–40 mW/cm2) on the liver mitochondria isolated from both the normal and type 2 diabetic rats. It was revealed that on the one hand the rate of oxygen absorption by mitochondria in rats with diabetes was decreased versus the quantity in rats of control group, but on the other hand the value was increased due to the LILI action. The respiratory data of control rats did not change after the of laser irradiation effect. The mitochondrial functions were decreased as the result of the VIRPEI influence. The respiratory processes are the basic ones for receiving the energy by various biological objects, both multicellular organisms and unicell ones, for example bacteria. Since the biological respiration is based on the electron transport chains functioning, isolated mitochondria is a model system for studying the respiratory processes. The application field of these effects is first of all biotechnology, particularly, cultivation of cells and tissues.
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