菲律宾2009-2015年诊断医学寄生虫学国家外部质量评估计划

Sherwin Galit, D. Tangcalagan, Julius Matt Rapanut, Alexander Sadiasa, Prince Ninja Borromeo Bautista, Kim Joshua Miranda Dominguez, J. Banaga, Celine Bernice Abaño Roxas, R. Navarro, Mark Philip Bugayong, J. Luchavez, M. Mondoy, L. Sombrero, S. Lupisan
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摘要

由菲律宾卫生部(DOH)授权的热带医学研究所(RITM) -疟疾和其他寄生虫国家参考实验室(NRL)通过国家外部质量评估计划(NEQAS),对菲律宾各地的临床实验室进行医学寄生虫诊断的年度能力测试(PT)。寄生虫学培训旨在监测和评估菲律宾实验室在鉴定血液和肠道寄生虫方面的能力,以及在疟疾感染的血膜中估计疟疾寄生虫密度的能力。截至2018年,参与NEQAS是卫生-卫生设施和服务监管局(DOH-HFSRB)对每个临床实验室获得运营许可证的年度要求。本报告旨在总结2009 - 2015年疟疾和粪便寄生虫显微检查的结果,并评估参与实验室在疟疾和粪便寄生虫显微检查方面的表现。方法2008年国家质量评价体系中以RITM-NRL为导向的临床实验室。实验室将填写好的报名表提交给RITM-NRL,并在2009年至2015年期间支付报名费用。参与的实验室鉴定了血膜中的疟疾种类和福尔马林保存的粪便标本中的寄生虫。并对疟原虫血膜中的寄生虫密度进行了估计。结果2009 - 2015年共有1440个实验室参与。这7年的平均和中位数得分都低于80分的分界点。日本血吸虫鉴定难度最大,仅有7.7%的实验室鉴定结果正确。2010年至2014年,大多数与会者给出的疟原虫密度估计值超出了可接受的范围。结论显微镜技术需要通过培训和进修课程来磨练,以提高和保持实验室人员在诊断寄生虫学方面的工作质量。
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The National External Quality Assessment Scheme for Diagnostic Medical Parasitology in the Philippines, 2009–2015
OBJECTIVE The Research Institute for Tropical Medicine (RITM)–National Reference Laboratory (NRL) for Malaria and Other Parasites, mandated by the Department of Health–Philippines (DOH), administers an annual Proficiency Test (PT) in diagnostic medical parasitology to clinical laboratories throughout the Philippines through the National External Quality Assessment Scheme (NEQAS). The PT in Parasitology aims to monitor and evaluate the capability of Philippine laboratories in the identification of blood and intestinal parasites, and the estimation of malaria parasite density in malaria-infected blood films. As of 2018, participation in the NEQAS is an annual requirement by the Department of Health–Health Facilities and Services Regulatory Bureau (DOH-HFSRB) for each clinical laboratory to obtain a license to operate. This report aims to summarize the results of the PT for Parasitology and assess the performance of participating laboratories in malaria and fecal parasite microscopy from 2009 to 2015. METHODOLOGY RITM–NRL oriented clinical laboratories in the NEQAS in 2008. Laboratories submitted their accomplished enrolment forms to RITM–NRL and paid fees to enroll in the PT in 2009 to 2015. Participating laboratories identified the species of malaria in blood films and the parasite/s in formalin-preserved fecal specimens. Estimation of parasite density in malaria blood films was performed as well. RESULTS One thousand five hundred forty laboratories participated in 2009 to 2015. Mean and median scores in all seven years were below the cut-off score of 80. Schistosoma japonicum was the most difficult to identify with  only 7.7% of laboratories having correct identification result.  Majority of participants in 2010 to 2014 gave malaria parasite density estimates outside the acceptable range. CONCLUSION Microscopy requires skill that is honed through training and refresher courses in order to enhance and maintain the quality of performance of laboratorians in diagnostic parasitology.
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