C Bonne, D Junquero, J Y Driot, C Coquelet, G Modat
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引用次数: 0
摘要
角膜内皮是类维生素a和表皮生长因子(EGF)的靶组织。我们在此报道维甲酸及其合成类似物CBS-211 A (10(-8)-10(-7) M)增强EGF对培养的牛角膜内皮细胞(BCEC)的有丝分裂作用。此外,类维甲酸治疗增加了BCEC的egf结合能力。与类维甲酸同时加入环己亚胺(0.5微克/毫升)可抑制类维甲酸对egf结合的影响。这些结果表明,类维生素a可以作为早期事件诱导BCEC上EGF受体的表达。本研究证实了类维甲酸和表皮生长因子在角膜内皮中的协同作用,提示类维甲酸可能对角膜内皮的组织修复有有益的作用。
[Effect of retinoids on the growth of corneal endothelial cells in culture].
Corneal endothelium is a target tissue for retinoids and epidermal growth factor (EGF). We report here that retinoic acid and its synthetic analog, CBS-211 A (10(-8)-10(-7) M), enhance the mitogenic effect of EGF on cultured bovine corneal endothelial cells (BCEC). Furthermore, retinoid treatment increases the EGF-binding capacity of BCEC. The addition of cycloheximide (0.5 micrograms/ml) to cultures simultaneously with retinoids suppressed the retinoid effect on EGF-binding. These results suggest that retinoids could induce the expression of EGF receptors on BCEC as an early event. This study demonstrates a cooperation between retinoids and EGF in corneal endothelium and suggests that retinoids could have a beneficial effect on this tissue repair in vivo.