姜黄(Curcuma Mangga Val. & Zijp)总黄酮含量、抗氧化活性及其化学计量学分类

Yuniar Intan Hartono, Indah Widyastuti, Hanna Zaidah Luthfah, Rosy Islamadina, A. Can, A. Rohman
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引用次数: 1

摘要

就生物多样性而言,印尼是一个富裕的国家。被广泛用于保持身体健康的草药来自姜科,包括铁木。姜黄(Curcuma mangga Val. & Zijp)能有效缓解胃痛、腹泻、经期疼痛、痤疮和溃疡,并能增加食欲。此外,由于其酚类和类黄酮化合物的存在,铁木被认为具有抗氧化活性。本研究旨在以总黄酮含量和抗氧化活性为指标,确定不同产地铁蹄草的相关性和分类。采用甲醇提取,AlCl3法测定总黄酮含量,DPPH自由基捕获法测定抗氧化活性。采用相关系数法和化学计量学主成分分析法(PCA)和聚类分析法(CA)分析总黄酮含量与其抗氧化活性之间的关系,并进行分类。芒草甲醇提取物总黄酮含量与其抗氧化活性呈中等相关性(r =-0.6085),通过主成分分析(PCA)和主成分分析(CA)成功聚类,将样品分为4组。关键词:姜黄,甲醇提取物,类黄酮,抗氧化剂,PCA, CA
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Total Flavonoid Content and Antioxidant Activity of Temu Mangga (Curcuma mangga Val. & Zijp) and its Classification with Chemometrics
Indonesia is a rich country in terms of its biodiversity. Herbs that are widely used tomaintain a healthy body comes from the Zingiberaceae family, including temu mangga. Temu mangga(Curcuma mangga Val. & Zijp) is effective for relieving gastric pain, diarrhea, menstrual pain, acneand ulcers, and increasing appetite. Besides, temu mangga is considered to have antioxidant activitydue to the presence of the phenolic and flavonoid compounds. This study aims to determine thecorrelation and classification of temu mangga from various places based on total flavonoid contentand antioxidant activities. The samples were extracted with methanol, then tested for totalflavonoid content by AlCl3 method and antioxidant activity by DPPH free radical capture method.Analysis was performed using correlation coefficient value and chemometrics PCA (PrincipalComponent Analysis) and CA (Cluster Analysis) to see the relationship between total flavonoidcontent with their antioxidant activity and to see their classification. Total flavonoid content ofmethanolic extract of temu mangga gives a moderate correlation to its antioxidant activity with r =-0.6085 and clustering samples successfully determined by PCA and CA which resulted in 4 groups.Keywords: Curcuma mangga Val. & Zijp, Methanolic Extract, Flavonoid, Antioxidant, PCA, CA
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