一种快速基因编码荧光传感器在小鼠、鱼类、蠕虫和苍蝇体内忠实检测乙酰胆碱

BiochemRN: Other Biochemistry Methods (Topic) Pub Date : 2020-03-30 DOI:10.2139/ssrn.3554080

P. Borden, Peng Zhang, Amol V. Shivange, J. Marvin, Joseph Cichon, Chuntao Dan, Kaspar Podgorski, Antonio Figueiredo, O. Novák, M. Tanimoto, E. Shigetomi, Mark A. Lobas, Hyun-Tae Kim, Paula K Zhu, Yajun Zhang, W. Zheng, Chengcheng Fan, Guangfu Wang, Bowen Xiang, Lihua Gan, Guang-Xian Zhang, Kaiming Guo, Li Lin, Yuan Cai, Andrew G Yee, Abhi Aggarwal, Huan Bao, Xiaochu Lou, E. Chapman, C. P. Ford, D. Rees, D. Dietrich, B. Khakh, J. Dittman, W. Gan, Minoru Koyama, V. Jayaraman, J. Cheer, H. Lester, J. J. Zhu, L. Looger

{"title":"一种快速基因编码荧光传感器在小鼠、鱼类、蠕虫和苍蝇体内忠实检测乙酰胆碱","authors":"P. Borden, Peng Zhang, Amol V. Shivange, J. Marvin, Joseph Cichon, Chuntao Dan, Kaspar Podgorski, Antonio Figueiredo, O. Novák, M. Tanimoto, E. Shigetomi, Mark A. Lobas, Hyun-Tae Kim, Paula K Zhu, Yajun Zhang, W. Zheng, Chengcheng Fan, Guangfu Wang, Bowen Xiang, Lihua Gan, Guang-Xian Zhang, Kaiming Guo, Li Lin, Yuan Cai, Andrew G Yee, Abhi Aggarwal, Huan Bao, Xiaochu Lou, E. Chapman, C. P. Ford, D. Rees, D. Dietrich, B. Khakh, J. Dittman, W. Gan, Minoru Koyama, V. Jayaraman, J. Cheer, H. Lester, J. J. Zhu, L. Looger","doi":"10.2139/ssrn.3554080","DOIUrl":null,"url":null,"abstract":"Here we design and optimize a genetically encoded fluorescent indicator, iAChSnFR, for the ubiquitous neurotransmitter acetylcholine, based on a bacterial periplasmic binding protein. iAChSnFR shows large fluorescence changes, rapid rise and decay kinetics, and insensitivity to most cholinergic drugs. iAChSnFR revealed large transients in a variety of slice and in vivo preparations in mouse, fish, fly and worm. iAChSnFR will be useful for the study of acetylcholine in all organisms.","PeriodicalId":286484,"journal":{"name":"BiochemRN: Other Biochemistry Methods (Topic)","volume":"154 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2020-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"A Fast Genetically Encoded Fluorescent Sensor for Faithful in vivo Acetylcholine Detection in Mice, Fish, Worms and Flies\",\"authors\":\"P. Borden, Peng Zhang, Amol V. Shivange, J. Marvin, Joseph Cichon, Chuntao Dan, Kaspar Podgorski, Antonio Figueiredo, O. Novák, M. Tanimoto, E. Shigetomi, Mark A. Lobas, Hyun-Tae Kim, Paula K Zhu, Yajun Zhang, W. Zheng, Chengcheng Fan, Guangfu Wang, Bowen Xiang, Lihua Gan, Guang-Xian Zhang, Kaiming Guo, Li Lin, Yuan Cai, Andrew G Yee, Abhi Aggarwal, Huan Bao, Xiaochu Lou, E. Chapman, C. P. Ford, D. Rees, D. Dietrich, B. Khakh, J. Dittman, W. Gan, Minoru Koyama, V. Jayaraman, J. Cheer, H. Lester, J. J. Zhu, L. Looger\",\"doi\":\"10.2139/ssrn.3554080\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Here we design and optimize a genetically encoded fluorescent indicator, iAChSnFR, for the ubiquitous neurotransmitter acetylcholine, based on a bacterial periplasmic binding protein. iAChSnFR shows large fluorescence changes, rapid rise and decay kinetics, and insensitivity to most cholinergic drugs. iAChSnFR revealed large transients in a variety of slice and in vivo preparations in mouse, fish, fly and worm. iAChSnFR will be useful for the study of acetylcholine in all organisms.\",\"PeriodicalId\":286484,\"journal\":{\"name\":\"BiochemRN: Other Biochemistry Methods (Topic)\",\"volume\":\"154 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-03-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BiochemRN: Other Biochemistry Methods (Topic)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2139/ssrn.3554080\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BiochemRN: Other Biochemistry Methods (Topic)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2139/ssrn.3554080","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 3

摘要

在这里，我们设计并优化了一种基于细菌周围质结合蛋白的遗传编码荧光指示剂，iAChSnFR，用于普遍存在的神经递质乙酰胆碱。iAChSnFR表现出较大的荧光变化，快速的上升和衰减动力学，对大多数胆碱能药物不敏感。iAChSnFR在小鼠、鱼、蝇和蠕虫的多种切片和体内制剂中均有较大的瞬变。iAChSnFR可用于研究所有生物体内的乙酰胆碱。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

A Fast Genetically Encoded Fluorescent Sensor for Faithful in vivo Acetylcholine Detection in Mice, Fish, Worms and Flies

Here we design and optimize a genetically encoded fluorescent indicator, iAChSnFR, for the ubiquitous neurotransmitter acetylcholine, based on a bacterial periplasmic binding protein. iAChSnFR shows large fluorescence changes, rapid rise and decay kinetics, and insensitivity to most cholinergic drugs. iAChSnFR revealed large transients in a variety of slice and in vivo preparations in mouse, fish, fly and worm. iAChSnFR will be useful for the study of acetylcholine in all organisms.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

BiochemRN: Other Biochemistry Methods (Topic)

自引率

0.00%

发文量