环状RNA hsa_circ_0000175通过miR-31-5p/GSDME轴作为类风湿关节炎的潜在生物标志物

IF 2.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemical Genetics Pub Date : 2023-11-15 DOI:10.1007/s10528-023-10576-6
Panpan Xin, Zhiming Tan, Zhiwen Wang, Yuhang Chen, Yu Zhuang
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引用次数: 0

摘要

类风湿性关节炎(RA)是一种常见的以滑膜炎症和关节损伤为特征的炎症性自身免疫性疾病。既往研究表明,焦亡在RA发病过程中起重要作用。本研究评价环状RNA hsa_circ0000175对类风湿关节炎焦亡和炎症的影响。RT-qPCR检测血清circ_0000175和miR-31-5p水平,Spearman相关分析评价两者之间的相关性。提取成纤维细胞样滑膜细胞(FLSs)并制备用于体外研究。FISH法检测circ_0000175的亚细胞定位。采用流式细胞术和ELISA法分别检测小鼠的焦亡率和炎症因子IL -1β、IL-18、IL-6。RNA下拉和荧光素酶报告基因检测证实了circ_0000175和miR-31-5p之间的相互作用。采用Western blot检测焦热相关因子(GSDME-N、GSDMD-N、cleaved caspase-1、cleaved caspase-3)的差异表达。Circ_0000175水平升高,而miR-31-5p在RA患者的PBMCs和LPS/ atp处理的FLSs中表达降低,并呈负相关。此外,miR-31-5p是RA-FLSs中circ_0000175的靶标。沉默circ_0000175或过表达miR-31-5p可通过caspase-1/GSDMD和caspase-3/GSDME途径显著减轻LPS/ atp诱导的FLSs焦亡。此外,GSDME被确定为miR-31-5p的靶标。敲低GSDME可增强circ_0000175对LPS/ATP处理的RA-FLSs的焦亡和炎症的抑制作用。Circ_0000175可通过miR-31-5p/GSDME轴在RA发生过程中诱导焦亡并引发炎症反应,为RA治疗提供了新的治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Circular RNA hsa_circ_0000175 Serves as a Potential Biomarker for Rheumatoid Arthritis via miR-31-5p/GSDME Axis
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来源期刊
Biochemical Genetics
Biochemical Genetics 生物-生化与分子生物学
CiteScore
3.90
自引率
0.00%
发文量
133
审稿时长
4.8 months
期刊介绍: Biochemical Genetics welcomes original manuscripts that address and test clear scientific hypotheses, are directed to a broad scientific audience, and clearly contribute to the advancement of the field through the use of sound sampling or experimental design, reliable analytical methodologies and robust statistical analyses. Although studies focusing on particular regions and target organisms are welcome, it is not the journal’s goal to publish essentially descriptive studies that provide results with narrow applicability, or are based on very small samples or pseudoreplication. Rather, Biochemical Genetics welcomes review articles that go beyond summarizing previous publications and create added value through the systematic analysis and critique of the current state of knowledge or by conducting meta-analyses. Methodological articles are also within the scope of Biological Genetics, particularly when new laboratory techniques or computational approaches are fully described and thoroughly compared with the existing benchmark methods. Biochemical Genetics welcomes articles on the following topics: Genomics; Proteomics; Population genetics; Phylogenetics; Metagenomics; Microbial genetics; Genetics and evolution of wild and cultivated plants; Animal genetics and evolution; Human genetics and evolution; Genetic disorders; Genetic markers of diseases; Gene technology and therapy; Experimental and analytical methods; Statistical and computational methods.
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