In-Sun Yu, Jungseok Choi, Mina K Kim, Min Jung Kim
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引用次数: 0
摘要
胎牛血清(FBS)含有多种营养成分,占细胞培养肉生长培养基的20%。然而,出于伦理、成本和科学方面的考虑,我们必须找到替代方案。在这项研究中,我们研究了商业制造的能够培养汉宇卫星细胞(HWSCs)的无血清培养基,以确定其增殖促进因子。选择6种不同的无血清培养基,将这些无血清培养基与添加20% FBS的对照培养基中的HWSC增殖率进行比较。在6种培养基中,只有StemFlexTM Medium (SF)和Mesenchymal Stem cell Growth Medium (DXF)的细胞增殖率高于对照培养基。SF和MS中含有高浓度的成纤维细胞生长因子2 (FGF2),我们发现在SF或MS中培养的细胞中FGF2蛋白表达上调,相关生物标志物(FGFR1、FRS2、Raf1、ERK、p38、Pax7和MyoD)的存在证实了成纤维细胞生长因子受体1 (FGFR1)介导的信号通路的激活和肌肉卫星细胞增殖相关因子的刺激,定量聚合酶链反应、western blotting、和免疫细胞化学。此外,FGFR1抑制剂PD173074抑制SF和MS的细胞增殖,下调相关生物标志物(FGFR1、FRS2、Raf1和ERK)。因此,SF和MS的细胞增殖促进归因于FGF2,这表明FGFR1在肌肉卫星细胞中的激活可能是提高细胞培养肉生产效率的目标。
The Comparison of Commercial Serum-Free Media for Hanwoo Satellite Cell Proliferation and the Role of Fibroblast Growth Factor 2.
Fetal bovine serum (FBS), which contains various nutrients, comprises 20% of the growth medium for cell-cultivated meat. However, ethical, cost, and scientific issues, necesitates identification of alternatives. In this study, we investigated commercially manufactured serum-free media capable of culturing Hanwoo satellite cells (HWSCs) to identify constituent proliferation enhancing factors. Six different serum-free media were selected, and the HWSC proliferation rates in these serum-free media were compared with that of control medium supplemented with 20% FBS. Among the six media, cell proliferation rates were higher only in StemFlexTM Medium (SF) and Mesenchymal Stem Cell Growth Medium DXF (MS) than in the control medium. SF and MS contain high fibroblast growth factor 2 (FGF2) concentrations, and we found upregulated FGF2 protein expression in cells cultured in SF or MS. Activation of the fibroblast growth factor receptor 1 (FGFR1)-mediated signaling pathway and stimulation of muscle satellite cell proliferation-related factors were confirmed by the presence of related biomarkers (FGFR1, FRS2, Raf1, ERK, p38, Pax7, and MyoD) as indicated by quantitative polymerase chain reaction, western blotting, and immunocytochemistry. Moreover, PD173074, an FGFR1 inhibitor suppressed cell proliferation in SF and MS and downregulated related biomarkers (FGFR1, FRS2, Raf1, and ERK). The promotion of cell proliferation in SF and MS was therefore attributed to FGF2, which indicates that FGFR1 activation in muscle satellite cells may be a target for improving the efficiency of cell-cultivated meat production.
期刊介绍:
Food Science of Animal Resources (Food Sci. Anim. Resour.) is an international, peer-reviewed journal publishing original research and review articles on scientific and technological aspects of chemistry, biotechnology, processing, engineering, and microbiology of meat, egg, dairy, and edible insect/worm products.