在韩国分布的动物资源加工食品中必必新和去甲必新的验证、测量不确定度及测定。

IF 4.2 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Food Science of Animal Resources Pub Date : 2023-11-01 DOI:10.5851/kosfa.2023.e49
Ga-Yeong Lee, Choong-In Yun, Juhee Cho, Young-Jun Kim
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引用次数: 0

摘要

本研究旨在验证一种高效液相色谱法定量测定各种食品中碧馨和去甲碧馨的方法。采用二极管阵列检测器(495 nm)技术。对方法的特异性、线性度、检出限、定量限、精密度、准确度进行验证,并对测量不确定度进行评定。在0.2 ~ 25 mg/L的检测浓度范围内,线性关系良好(r2≥0.9999)。必必新和去甲必新的定量限为0.03 ~ 0.11 mg/L,定量限为0.02 ~ 0.05 mg/L。必必新的日内、日间精密度和相对SD (RSD)分别为88.0±1.3 ~ 97.0±0.5%和0.2% ~ 2.6%,去甲必新的日内、日间精密度和相对SD (RSD)分别为88.2±0.8 ~ 105.8±0.8%和0.3% ~ 2.7%。在三个实验室进行了准确度和精密度的实验室间验证,结果均符合AOAC指南。此外,相对扩展不确定度(
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Validation, Measurement Uncertainty, and Determination of Bixin and Norbixin in Processed Foods of Animal Resources Distributed in Korea.

This research aimed to validate a high-performance liquid chromatography method for the quantitative determination of bixin and norbixin in various foods. The Diode Array Detector (495 nm) technique was used. Method was validated for specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy, and the measurement uncertainty was assessed. The calibration curve showed excellent linearity (r2≥0.9999) over the tested concentration range of 0.2-25 mg/L. The LOD and LOQ were 0.03-0.11 and 0.02-0.05 mg/L for bixin and norbixin, respectively. The intra- and inter-day accuracies and precisions were 88.0±1.3-97.0±0.5% and 0.2%-2.6% relative SD (RSD) for bixin and 88.2±0.8-105.8±0.8% and 0.3%-2.7% RSD for norbixin, respectively. Inter-laboratory validation for accuracy and precision was conducted in three laboratories, and these results all met the AOAC guidelines. In addition, the relative expanded uncertainty (<22%) satisfied the CODEX recommendation. Furthermore, products distributed in Korea were monitored for annatto extracts using the proposed method to demonstrate its application. The developed analytical method is reliable for quantifying bixin and norbixin in various foods.

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来源期刊
Food Science of Animal Resources
Food Science of Animal Resources Agricultural and Biological Sciences-Animal Science and Zoology
CiteScore
6.70
自引率
6.70%
发文量
75
期刊介绍: Food Science of Animal Resources (Food Sci. Anim. Resour.) is an international, peer-reviewed journal publishing original research and review articles on scientific and technological aspects of chemistry, biotechnology, processing, engineering, and microbiology of meat, egg, dairy, and edible insect/worm products.
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