聚合平板秘密转化生长因子α诱导的小鼠肉瘤PC-103细胞。

Eksperimental'naia onkologiia Pub Date : 1990-01-01
A A Stavrovskaia, T P Stromskaia, R M Brodskaia, R S Stoĭka, S I Sushel'nitskiĭ, S I Garas'ko, G S Shafranskaia
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引用次数: 0

摘要

先前研究表明,塑料薄膜皮下移植诱导的小鼠肉瘤PS-103细胞具有促生长活性。本文对从PS-103肉瘤中分离的3sb进行了研究。该克隆细胞产生的生长因子在单层和半固体培养基中均能刺激准正常细胞(3T3、NRK)和转化细胞的增殖。凝胶过滤层析表明,主要的促生长活性随蛋白组分m.m 10- 15kda迁移。在培养基中添加该生长因子可显著(90%)抑制A-431细胞对125I-EGF(表皮生长因子)的结合。这些数据表明,所研究的肿瘤细胞产生的生长因子是转化生长因子。
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[The cells of murine sarcoma PC-103 induced by a polymeric plate secret transforming growth factor alpha].

It was shown previously that cells of sarcoma PS-103 induced in mouse by subcutaneous transplantation of plastic film produce growth-stimulating activity. In this communication clone 3 sb, isolated from sarcoma PS-103, was studied. Growth factor produced by the cells of this clone stimulated proliferation of quasi-normal (3T3, NRK) cells and transformed cells both in monolayer and in semi-solid medium. It was shown by gel filtration chromatography that the main growth-stimulating activity migrated with the protein fraction m.m. 10-15 kDa. The addition of this growth factor to the culture medium significantly (90%) inhibited 125I-EGF (epidermal growth factor) binding by A-431 cells. These data suggest that the growth factor produced by the studied tumor cells is transforming growth factor alpha.

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