Hishar Mirsam, None Suriani, Sri Kurniawati, Oky Dwi Purwanto, Amran Muis, Syahrir Pakki, A. Tenrirawe, Nurnina Nonci, None Herawati, None Muslimin, Muhammad Azrai
{"title":"玉米曲霉木霉对带状叶枯病和鞘枯病的体外抑制机制及其对玉米幼苗生长的促进作用","authors":"Hishar Mirsam, None Suriani, Sri Kurniawati, Oky Dwi Purwanto, Amran Muis, Syahrir Pakki, A. Tenrirawe, Nurnina Nonci, None Herawati, None Muslimin, Muhammad Azrai","doi":"10.1186/s41938-023-00729-5","DOIUrl":null,"url":null,"abstract":"Abstract Background One of the primary corn diseases, banded leaf and sheath blight, is carried on by the pathogenic fungus Rhizoctonia solani . Efforts to control R. solani are more directed at environmentally friendly control using a biological control agent, such as Trichoderma spp. as antagonistic agents and plant growth promoter. This study aimed to identify T. asperellum isolates based on molecular characteristics and to determine the in vitro inhibition mechanism against R. solani and its role in enhancing the growth of corn seedlings. Results The HMRP7, HMRF7A, HMEDF1B, HMEDF6A, and CHM01 isolates were identified as T. asperellum with a genetic distance coefficient value of 0.000 and a very high similarity of 100%. Meanwhile, the RsHM isolate was identified as R. solani with a homology level of > 90% and genetic distance coefficient values ranging from 0.000 to 0.032. The antagonistic ability showed that the five T. asperellum isolates were able to inhibit the growth of R. solani in vitro on PDA medium with an inhibition percentage of ≥ 50%, so they were categorized as antagonist agents. T. asperellum showed the antagonistic mechanism in inhibiting the R. solani growth through the action of parasitism. The five T. asperellum isolates tested on corn seedlings showed a significantly high difference from the control treatment on the observational variables of maximum growth potential (MGP), growth rate (GtR), growth simultaneity (GS), vigor index (VI), germination rate (GR), and median germination time (T50). Conclusion CHM01 isolate showed better potential than other isolates in inhibiting the growth of R. solani in vitro on PDA medium with a parasitism mechanism and enhancing the growth of corn seedlings.","PeriodicalId":11514,"journal":{"name":"Egyptian Journal of Biological Pest Control","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"In vitro inhibition mechanism of Trichoderma asperellum isolates from corn against Rhizoctonia solani causing banded leaf and sheath blight disease and its role in improving the growth of corn seedlings\",\"authors\":\"Hishar Mirsam, None Suriani, Sri Kurniawati, Oky Dwi Purwanto, Amran Muis, Syahrir Pakki, A. Tenrirawe, Nurnina Nonci, None Herawati, None Muslimin, Muhammad Azrai\",\"doi\":\"10.1186/s41938-023-00729-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract Background One of the primary corn diseases, banded leaf and sheath blight, is carried on by the pathogenic fungus Rhizoctonia solani . Efforts to control R. solani are more directed at environmentally friendly control using a biological control agent, such as Trichoderma spp. as antagonistic agents and plant growth promoter. This study aimed to identify T. asperellum isolates based on molecular characteristics and to determine the in vitro inhibition mechanism against R. solani and its role in enhancing the growth of corn seedlings. Results The HMRP7, HMRF7A, HMEDF1B, HMEDF6A, and CHM01 isolates were identified as T. asperellum with a genetic distance coefficient value of 0.000 and a very high similarity of 100%. Meanwhile, the RsHM isolate was identified as R. solani with a homology level of > 90% and genetic distance coefficient values ranging from 0.000 to 0.032. The antagonistic ability showed that the five T. asperellum isolates were able to inhibit the growth of R. solani in vitro on PDA medium with an inhibition percentage of ≥ 50%, so they were categorized as antagonist agents. T. asperellum showed the antagonistic mechanism in inhibiting the R. solani growth through the action of parasitism. The five T. asperellum isolates tested on corn seedlings showed a significantly high difference from the control treatment on the observational variables of maximum growth potential (MGP), growth rate (GtR), growth simultaneity (GS), vigor index (VI), germination rate (GR), and median germination time (T50). Conclusion CHM01 isolate showed better potential than other isolates in inhibiting the growth of R. solani in vitro on PDA medium with a parasitism mechanism and enhancing the growth of corn seedlings.\",\"PeriodicalId\":11514,\"journal\":{\"name\":\"Egyptian Journal of Biological Pest Control\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2023-09-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Egyptian Journal of Biological Pest Control\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1186/s41938-023-00729-5\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Egyptian Journal of Biological Pest Control","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s41938-023-00729-5","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
In vitro inhibition mechanism of Trichoderma asperellum isolates from corn against Rhizoctonia solani causing banded leaf and sheath blight disease and its role in improving the growth of corn seedlings
Abstract Background One of the primary corn diseases, banded leaf and sheath blight, is carried on by the pathogenic fungus Rhizoctonia solani . Efforts to control R. solani are more directed at environmentally friendly control using a biological control agent, such as Trichoderma spp. as antagonistic agents and plant growth promoter. This study aimed to identify T. asperellum isolates based on molecular characteristics and to determine the in vitro inhibition mechanism against R. solani and its role in enhancing the growth of corn seedlings. Results The HMRP7, HMRF7A, HMEDF1B, HMEDF6A, and CHM01 isolates were identified as T. asperellum with a genetic distance coefficient value of 0.000 and a very high similarity of 100%. Meanwhile, the RsHM isolate was identified as R. solani with a homology level of > 90% and genetic distance coefficient values ranging from 0.000 to 0.032. The antagonistic ability showed that the five T. asperellum isolates were able to inhibit the growth of R. solani in vitro on PDA medium with an inhibition percentage of ≥ 50%, so they were categorized as antagonist agents. T. asperellum showed the antagonistic mechanism in inhibiting the R. solani growth through the action of parasitism. The five T. asperellum isolates tested on corn seedlings showed a significantly high difference from the control treatment on the observational variables of maximum growth potential (MGP), growth rate (GtR), growth simultaneity (GS), vigor index (VI), germination rate (GR), and median germination time (T50). Conclusion CHM01 isolate showed better potential than other isolates in inhibiting the growth of R. solani in vitro on PDA medium with a parasitism mechanism and enhancing the growth of corn seedlings.
期刊介绍:
The Egyptian Journal of Biological Pest Control is a periodic scientific journal published by the Egyptian Society for Biological Control of Pests (ESBCP) in collaboration with SpringerNature. The journal aims to publish internationally peer-reviewed, high-quality research articles in the field of biological and integrated pest control (non-chemical control). The journal publishes review articles, original papers, conference reports, book reviews, editorials, laboratory reports, technical notes and short communications.