一种在摇瓶中微生物培养过程中协调流体动力的程序

IF 1.6 Q2 EDUCATION, SCIENTIFIC DISCIPLINES Journal of Microbiology & Biology Education Pub Date : 2023-09-19 DOI:10.1128/jmbe.00099-23
Lúcia Chaves Simões, Isabel Oliveira, Anabela Borges, Inês Bezerra Gomes, Manuel Simões
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引用次数: 0

摘要

摇瓶培养是微生物学和生物技术实验室的一项常规技术,细胞生长受到流体动力条件的影响,这取决于搅拌速度、摇瓶直径和摇瓶大小。液体搅拌本质上是为了增加通气,底物转移到细胞,防止沉淀,而忽略了流体动力学在微生物生长和代谢中的作用。在这里,我们提出了一种简单的方法来帮助标准化轨道激振器中的流体动力,以提高实验的准确性和重复性,并让学生更好地了解搅拌过程在微生物生长中的意义。
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A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks
ABSTRACT Shake flask cultivation is a routine technique in microbiology and biotechnology laboratories where cell growth can be affected by the hydrodynamic conditions, which depend on the agitation velocity, shaking diameter, and shake flask size. Liquid agitation is implemented inherently to increase aeration, substrate transfer to the cells, and prevent sedimentation, disregarding the role of hydrodynamics in microbial growth and metabolism. Here, we present a simple approach to help standardize the hydrodynamic forces in orbital shakers to increase the experimental accuracy and reproducibility and give students a better knowledge of the significance of the agitation process in microbial growth.
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来源期刊
Journal of Microbiology & Biology Education
Journal of Microbiology & Biology Education EDUCATION, SCIENTIFIC DISCIPLINES-
CiteScore
3.00
自引率
26.30%
发文量
95
审稿时长
22 weeks
期刊最新文献
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