基于光子晶体竞争免疫分析法的单液滴唾液检测用于偏头痛的精确诊断

SmartMat Pub Date : 2023-11-10 DOI:10.1002/smm2.1252
Xiaoxue Lin, Jimei Chi, Zewei Lian, Yang Yun, Xu Yang, Xuwei He, Zheng Liu, Shuqing Wang, Wei Zhao, Zihua Gong, Yingyuan Liu, Shuhua Zhang, Deqi Zhai, Siyuan Xie, Yin Sun, Meng Su, Zhao Dong, Shengyuan Yu, Yanlin Song
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引用次数: 0

摘要

偏头痛在世界范围内普遍存在。目前的诊断标准完全依赖于临床特征,没有任何客观可靠的手段。降钙素基因相关肽(CGRP)作为鉴别偏头痛的生物标志物,其降解迅速,半衰期小于10分钟,这对临床应用中CGRP的护理点检测提出了重大挑战。本研究开发了一种基于光子晶体(PC)的生物芯片,通过荧光竞争法检测CGRP。该芯片集成了荧光增强和亲疏水模式富集功能,能够快速、灵敏地检测CGRP。通过对pc附近荧光的最佳增强距离进行研究,该芯片可在室温下使用<30 μL唾液在10 min内检测CGRP,最低检测限为0.05 pg/mL。此外,我们还利用PC生物芯片检测了70名受试者唾液中的CGRP浓度。结果与酶联免疫吸附试验(ELISA)具有很强的一致性,线性相关系数r2为0.97。这种在如此短的时间内对标记物的敏感检测超过了ELISA的能力,这为建立偏头痛临床表型和生物标记物的精确诊断框架铺平了道路。
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One‐droplet saliva detection on photonic crystal‐based competitive immunoassay for precise diagnosis of migraine
Abstract Migraine exhibits a substantial prevalence worldwide. The current diagnostic criteria rests exclusively on clinical characteristics without any objective and reliable means. The calcitonin gene‐related peptide (CGRP), as a biomarker for distinguishing migraine, undergoes swift degradation, featuring a half‐life of under 10 min, which poses a significant challenge to the point‐of‐care testing of CGRP in clinical application. Here, a photonic crystal (PC)‐based biochip has been developed to detect CGRP via the fluorescence competition assay. The chip integrates the functionalities of fluorescence enhancement and hydrophilic–hydrophobic patterning enrichment, enabling rapid and sensitive detection of CGRP. After investigating the optimal enhancement distance of fluorescence near PCs, the chip allows CGRP detection using <30 μL of saliva at room temperature within 10 min. A minimum detection limit of 0.05 pg/mL is achieved. Furthermore, CGRP concentrations in the saliva of 70 subjects have been tested by PC biochips. The results exhibit strong concordance with the enzyme‐linked immunosorbent assay (ELISA), demonstrating a linear correlation coefficient of R 2 of 0.97. This sensitive detection of markers within such a short duration surpasses the capacities of ELISA, which paves the way for establishing a precise diagnostic framework integrating clinical phenotypes and biomarkers for migraine.
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