{"title":"β-2微球蛋白和胱抑素C作为小儿HIV感染局灶节段性肾小球硬化的尿液生物标志物的作用","authors":"K Persad, L Nandlal, R Bhimma, T Naicker","doi":"10.7196/sajch.2023.v17i2.1951","DOIUrl":null,"url":null,"abstract":"Background. Africa has the highest rate of HIV infection, and HIV-associated nephropathy (HIVAN) is one of the most frequent kidneydiseases observed in children. HIVAN in children usually presents as a form of nephrotic syndrome, predominantly focal segmentalglomerulosclerosis (FSGS) on histopathology, that often leads to chronic kidney failure.Objective. This study determined the urinary concentrations of β-2-microglobulin (β2M) and cystatin C proteins in children with HIVANand primary FSGS.Methods. The study group comprised 34 children; 14 with HIVAN and 20 with primary FSGS. The control groups were 20 HIV-positiveand 20 HIV-negative children with no kidney disease. Urine samples collected from these 74 children were stored at -80°C. Bio-Plextechnology was used to analyse the urinary protein concentration of cystatin C and β2M.Results. A significant increase in urinary β2M levels was observed in the HIVAN group compared with the HIV-negative group(p=0.0240). No other statistically significant differences in urinary β2M concentrations were noted across the study groups. Urinarycystatin C levels were significantly increased in primary FSGS children compared with both HIV-negative (p=0.0041) and HIV-positivecontrols (p=0.0256). Urinary cystatin C displayed a significant increase in the primary FSGS compared with the HIVAN group (p=0.0150).No significant differences in urinary cystatin C levels were noted in the HIVAN group compared with the HIV-negative and HIV-positivecontrol groups.Conclusion. Urinary cystatin C has promising prognostic value to predict primary FSGS from HIVAN.","PeriodicalId":44732,"journal":{"name":"South African Journal of Child Health","volume":null,"pages":null},"PeriodicalIF":0.2000,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The role of β-2-microglobulin and cystatin C as urinary biomarkers of focal segmental glomerulosclerosis in the setting of paediatric HIV infection\",\"authors\":\"K Persad, L Nandlal, R Bhimma, T Naicker\",\"doi\":\"10.7196/sajch.2023.v17i2.1951\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background. Africa has the highest rate of HIV infection, and HIV-associated nephropathy (HIVAN) is one of the most frequent kidneydiseases observed in children. HIVAN in children usually presents as a form of nephrotic syndrome, predominantly focal segmentalglomerulosclerosis (FSGS) on histopathology, that often leads to chronic kidney failure.Objective. This study determined the urinary concentrations of β-2-microglobulin (β2M) and cystatin C proteins in children with HIVANand primary FSGS.Methods. The study group comprised 34 children; 14 with HIVAN and 20 with primary FSGS. The control groups were 20 HIV-positiveand 20 HIV-negative children with no kidney disease. Urine samples collected from these 74 children were stored at -80°C. Bio-Plextechnology was used to analyse the urinary protein concentration of cystatin C and β2M.Results. A significant increase in urinary β2M levels was observed in the HIVAN group compared with the HIV-negative group(p=0.0240). No other statistically significant differences in urinary β2M concentrations were noted across the study groups. Urinarycystatin C levels were significantly increased in primary FSGS children compared with both HIV-negative (p=0.0041) and HIV-positivecontrols (p=0.0256). Urinary cystatin C displayed a significant increase in the primary FSGS compared with the HIVAN group (p=0.0150).No significant differences in urinary cystatin C levels were noted in the HIVAN group compared with the HIV-negative and HIV-positivecontrol groups.Conclusion. Urinary cystatin C has promising prognostic value to predict primary FSGS from HIVAN.\",\"PeriodicalId\":44732,\"journal\":{\"name\":\"South African Journal of Child Health\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.2000,\"publicationDate\":\"2023-05-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"South African Journal of Child Health\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.7196/sajch.2023.v17i2.1951\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PEDIATRICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"South African Journal of Child Health","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.7196/sajch.2023.v17i2.1951","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PEDIATRICS","Score":null,"Total":0}
The role of β-2-microglobulin and cystatin C as urinary biomarkers of focal segmental glomerulosclerosis in the setting of paediatric HIV infection
Background. Africa has the highest rate of HIV infection, and HIV-associated nephropathy (HIVAN) is one of the most frequent kidneydiseases observed in children. HIVAN in children usually presents as a form of nephrotic syndrome, predominantly focal segmentalglomerulosclerosis (FSGS) on histopathology, that often leads to chronic kidney failure.Objective. This study determined the urinary concentrations of β-2-microglobulin (β2M) and cystatin C proteins in children with HIVANand primary FSGS.Methods. The study group comprised 34 children; 14 with HIVAN and 20 with primary FSGS. The control groups were 20 HIV-positiveand 20 HIV-negative children with no kidney disease. Urine samples collected from these 74 children were stored at -80°C. Bio-Plextechnology was used to analyse the urinary protein concentration of cystatin C and β2M.Results. A significant increase in urinary β2M levels was observed in the HIVAN group compared with the HIV-negative group(p=0.0240). No other statistically significant differences in urinary β2M concentrations were noted across the study groups. Urinarycystatin C levels were significantly increased in primary FSGS children compared with both HIV-negative (p=0.0041) and HIV-positivecontrols (p=0.0256). Urinary cystatin C displayed a significant increase in the primary FSGS compared with the HIVAN group (p=0.0150).No significant differences in urinary cystatin C levels were noted in the HIVAN group compared with the HIV-negative and HIV-positivecontrol groups.Conclusion. Urinary cystatin C has promising prognostic value to predict primary FSGS from HIVAN.