不同物候期Bt棉不同植株部位的Cry毒素表达

IF 2.1 3区 农林科学 Q1 ENTOMOLOGY Egyptian Journal of Biological Pest Control Pub Date : 2023-09-29 DOI:10.1186/s41938-023-00742-8
P. Likhitha, D. B. Undirwade, U. S. Kulkarni, A. V. Kolhe, M. P. Moharil
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Variation in the expression levels of delta endotoxins in different plant parts was one of the contributing factors to the survival of pest populations on Bt cotton. Results Using a commercially available QL 96 ELISA plate kit, the concentration of delta endotoxin in various plant parts at different phenological stages was determined in twelve BG-II cotton hybrids, namely Ajeet-155, JKCH-2245, RCH-3863, NCS-866, MRC-7373, JKCH-99, MRC-7387, NCEH-21, ANKR-3324, NCSI-1904, and NCHB 9902. Cry1Ac and Cry2Ab protein levels were determined from samples of flowers and fruiting parts (Rind, locule, seed, locule wall and seed) at 40, 75, 100 and 125 days after sowing (DAS) over two consecutive years 2018–2019 and 2019–2020. Cry1Ac protein content and expression was the highest at 100DAS in locule, seed and rind; followed by rind and locule wall and seed in green bolls at 125 DAS; followed by seed, locule, rind and flowers at 75 DAS; followed by flowers at 40 DAS in all the tested twelve Bollgard-II hybrids. Cry2Ab protein content and expression was the highest at 125DAS in locule wall and seed and rind, followed by seed, locule and rind in green bolls at 100 DAS, followed by seed, locule, rind and flowers at 75 DAS, then by flowers at 40 DAS in all the tested twelve Bollgard-II hybrids. Cry1Ac protein expression was less in comparison with Cry2Ab. Conclusions The research findings indicate that the locule of BG-II cotton plants exhibited the highest expression levels of Cry1Ac and Cry2Ab proteins, followed by the seeds, rind, locule wall, and flowers. 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引用次数: 0

摘要

背景与bollgard - 1相比,Cry2Ab蛋白在bollgard - 2棉花品种中的应用增强了对昆虫的控制。田间和实验室研究表明,与bollgard - 1和非bollgard棉花品种相比,Bollgard-II棉花品系棉铃虫侵染的终端、方形和铃数减少。这表明Cry2Ab与Cry1Ac的结合增加了蛋白质的整体表达。Cry蛋白在植物不同部位的表达存在差异,如叶片、苞片、方片和棉铃。随着季节的进展,Cry蛋白在这些植物部位的表达减少。叶片中Cry蛋白表达量最高,其次是方块、花和棉铃。不同部位δ内毒素表达水平的差异是影响Bt棉害虫种群存活的因素之一。结果采用市售的QL 96酶联免疫吸附测定试剂盒,测定了12个kg - ii型棉花杂交种Ajeet-155、JKCH-2245、RCH-3863、NCS-866、MRC-7373、JKCH-99、MRC-7387、NCEH-21、ANKR-3324、NCSI-1904和NCHB 9902不同物候阶段各部位的δ内毒素浓度。在2018-2019和2019-2020连续两年,分别于播种后40、75、100和125天从花和果部(果皮、果室、种子、果室壁和种子)样品中测定Cry1Ac和Cry2Ab蛋白水平。在果室、种子和果皮中Cry1Ac蛋白含量和表达量在100DAS时最高;其次是125 DAS绿铃的果皮、室壁和种子;其次是75 DAS的种子、子房室、果皮和花;然后是所有12个Bollgard-II杂交种在40 DAS时的花。12个bollard - ii型杂交种的Cry2Ab蛋白含量和表达量在125DAS时最高,在100 DAS时绿铃的种子、果室和果皮次之,在75 DAS时种子、果室、果皮和花次之,在40 DAS时花次之。Cry1Ac蛋白表达量低于Cry2Ab。结论g - ii型棉花胞室Cry1Ac和Cry2Ab蛋白表达量最高,其次为种子、果皮、胞室壁和花。这些结果为了解Cry蛋白在不同植物部位的表达分布提供了有价值的见解,有助于更好地了解Bt棉花品种的虫害防治。
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Cry toxin expression in different plant parts of Bt cotton at different phenological stages
Abstract Background Compared to Bollgard-I, the utilization of Cry2Ab protein in Bollgard-II cotton cultivars enhances insect control. Field and laboratory studies demonstrated reduction in the numbers of bollworm-infested terminals, squares, and bolls in Bollgard-II cotton lines when compared to both Bollgard-I and non-Bollgard cotton cultivars. This indicates that the combination of Cry2Ab with Cry1Ac increased the overall expression of proteins. The expression of Cry protein varied across different plant parts, such as leaves, bracts, squares, and bolls. As the season progresses, the expression of Cry protein decreased in these plant parts. Leaves exhibited the highest levels of Cry protein expression, followed by squares, flowers, and bolls. Variation in the expression levels of delta endotoxins in different plant parts was one of the contributing factors to the survival of pest populations on Bt cotton. Results Using a commercially available QL 96 ELISA plate kit, the concentration of delta endotoxin in various plant parts at different phenological stages was determined in twelve BG-II cotton hybrids, namely Ajeet-155, JKCH-2245, RCH-3863, NCS-866, MRC-7373, JKCH-99, MRC-7387, NCEH-21, ANKR-3324, NCSI-1904, and NCHB 9902. Cry1Ac and Cry2Ab protein levels were determined from samples of flowers and fruiting parts (Rind, locule, seed, locule wall and seed) at 40, 75, 100 and 125 days after sowing (DAS) over two consecutive years 2018–2019 and 2019–2020. Cry1Ac protein content and expression was the highest at 100DAS in locule, seed and rind; followed by rind and locule wall and seed in green bolls at 125 DAS; followed by seed, locule, rind and flowers at 75 DAS; followed by flowers at 40 DAS in all the tested twelve Bollgard-II hybrids. Cry2Ab protein content and expression was the highest at 125DAS in locule wall and seed and rind, followed by seed, locule and rind in green bolls at 100 DAS, followed by seed, locule, rind and flowers at 75 DAS, then by flowers at 40 DAS in all the tested twelve Bollgard-II hybrids. Cry1Ac protein expression was less in comparison with Cry2Ab. Conclusions The research findings indicate that the locule of BG-II cotton plants exhibited the highest expression levels of Cry1Ac and Cry2Ab proteins, followed by the seeds, rind, locule wall, and flowers. These results provide valuable insights into the distribution of Cry protein expression in different plant parts, which can contribute to a better understanding of insect control in Bt cotton cultivars.
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来源期刊
CiteScore
4.90
自引率
16.70%
发文量
128
审稿时长
>12 weeks
期刊介绍: The Egyptian Journal of Biological Pest Control is a periodic scientific journal published by the Egyptian Society for Biological Control of Pests (ESBCP) in collaboration with SpringerNature. The journal aims to publish internationally peer-reviewed, high-quality research articles in the field of biological and integrated pest control (non-chemical control). The journal publishes review articles, original papers, conference reports, book reviews, editorials, laboratory reports, technical notes and short communications.
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