含有甘氨酸的蜂胶水醇溶液对绿叶蔬菜中微生物和蠕虫卵联合还原的评价

Q3 Agricultural and Biological Sciences Brazilian Journal of Food Technology Pub Date : 2023-01-01 DOI:10.1590/1981-6723.04223
Juliana da Silveira, Natália de Almeida Corrêa Alfredo, Marcia Regina Beux, Sila Mary Rodrigues Ferreira, Diego Averaldo Guiguet Leal
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引用次数: 0

摘要

摘要本研究的主要目的是评价蜂胶水酒精溶液与甘氨酸对生菜叶片微生物的减少和蠕虫卵的去除效果。试验分为微生物组(ME)、寄生虫学组(PE)和微生物与寄生虫学组(MPE) 3个重复或重复。在实验之前,使用传统的微生物学技术,通过对中温需氧菌、霉菌和酵母的计数来评估原生微生物群的初始浓度。生菜叶在自来水中洗涤,并进行不同的浸泡处理:蜂胶水醇溶液(PHS),蜂胶水醇溶液pH为5.5 (PHS2),蜂胶水醇溶液甘氨酸(PHS-甘氨酸)。在ME试验中,对蜂胶水醇溶液与甘氨酸混合或不混合对原生微生物群减少的影响进行了评价。评估残留微生物污染。在本试验中,评价了PHS2和phs -甘氨酸对人工污染叶片上已知数量的蛔虫卵的去除效果。在MPE中,phs -甘氨酸对本地微生物群的减少和鸡蛋的去除进行了评估。在ME中观察到超过2 log CFU/g的减少。在PE中,phs -甘氨酸去除37.22%的卵子。在MPE中,phs -甘氨酸减少了3.3 CFU/g的中温需氧菌、5.0 log CFU/g的真菌和35.53%的蔬菜鸡蛋。结果强调了该解决方案在减少或消除蔬菜中不同污染物方面的效率,并强调有必要开发不损害人体健康并可在家庭一级重复使用的卫生方法。
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Evaluation of a propolis hydroalcoholic solution with glycine in the joint reduction of microorganisms and helminth eggs from leafy green vegetables
Abstract The main goal of this study was to evaluate the effectiveness of a propolis hydroalcoholic solution with glycine on the reduction of microorganisms and helminth eggs removal in lettuce leaves. Three experimental groups were evaluated in triplicate or replicates: microbiological (ME), parasitological (PE), and microbiological and parasitological (MPE). Before the experiments, the initial concentration of the native microbiota was assessed by counting mesophilic aerobes, molds and yeasts, using conventional microbiological techniques. Lettuce leaves were washed in tap water and subjected to different immersion treatments: propolis hydroalcoholic solution (PHS), propolis hydroalcoholic solution at pH 5.5 (PHS2), and propolis hydroalcoholic solution with glycine (PHS-glycine). In ME, propolis hydroalcoholic solution combined or not with glycine, was evaluated on the reduction of native microbiota. Residual microbiological contamination was assessed. In the PE, the effect of PHS2 and PHS-glycine was evaluated on the removal of a known number of Ascaris suum eggs on leaves artificially contaminated. In MPE, PHS-glycine was evaluated on the reduction of native microbiota and removal of eggs. A reduction of more than 2 log CFU/g was observed in ME. In PE, PHS-glycine removed 37.22% of eggs. In MPE, the PHS-glycine reduced 3.3 CFU/g of mesophilic aerobes, 5.0 log CFU/g of fungi, and 35.53% of eggs from vegetables. The results highlighted the solution’s efficiency in reducing or eliminating different contaminants in vegetables and reinforced the need for the development of hygiene methods that do not harm human health and are reproducible at the household level.
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来源期刊
Brazilian Journal of Food Technology
Brazilian Journal of Food Technology Agricultural and Biological Sciences-Food Science
CiteScore
2.20
自引率
0.00%
发文量
32
审稿时长
35 weeks
期刊介绍: The Brazilian Journal of Food Technology (BJFT) is an electronic rolling pass publication with free access, whose purpose is to publish unpublished articles based on original research results and technological information that significantly contribute to the dissemination of new knowledge related to production and evaluation of food in the areas of science, technology, food engineering and nutrition (non-clinical). Manuscripts of national or international scope are accepted, presenting new concepts or experimental approaches that are not only repositories of scientific data. The Journal publishes original articles, review articles, scientific notes, case reports, and short communication in Portuguese and English. The submission of a manuscript presupposes that the same paper is not under analysis for publication in any other divulging vehicle. Articles specifically contemplating analytical methodologies will be accepted as long as they are innovative or provide significant improvement to existing methods. It is at the discretion of the Editors, depending on the subject relevance, the acceptance of works with test results of industrialized products without the information necessary to manufacture them. Papers aimed essentially at commercial propaganda will not be accepted.
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