变化的颜色和理解:利用突变的显色蛋白和大肠杆菌的信息抑制菌株探索分子生物学的中心法则

IF 1.6 Q2 EDUCATION, SCIENTIFIC DISCIPLINES Journal of Microbiology & Biology Education Pub Date : 2023-10-25 DOI:10.1128/jmbe.00094-23
Sarah DeWolf, Maartje Van den Bogaard, Rachael Brady Hart, Sparrow Hartman, Nancy Boury, Gregory J. Phillips
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引用次数: 0

摘要

分子生物学的中心法则是生命科学本科生的一个关键概念,因为它描述了生命系统中从基因到基因产物的信息流动。然而,尽管在许多生命科学入门课程中经常涉及到,学生们可能仍然对中心教条有误解,即使他们进入高级课程。主动学习策略,如实验室活动,在解决这些误解方面是有用的。在这里展示的实验室练习中,高年级本科生探索无义抑制突变的复杂性,以挑战他们对中心教条的理解。学生们将携带无功能显色蛋白基因的质粒引入大肠杆菌的各种无义抑制菌株中,这是由于编码发色团的密码子发生了无义突变。然后学生观察不同的显色表型,这取决于抑制菌株。学生们对中心教条的理解有了适度的提高。虽然中心教条仍然是一个具有挑战性的概念,但像这里介绍的这样的主动学习策略可以帮助减少概念错误。
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Changing colors and understanding: the use of mutant chromogenic protein and informational suppressor strains of Escherichia coli to explore the central dogma of molecular biology
ABSTRACT The central dogma of molecular biology is a key concept for undergraduate students in the life sciences as it describes the flow of information in living systems from gene-to-gene product. However, despite often being covered in many introductory life science courses, students may still have misconceptions surrounding the central dogma even as they move on to advanced courses. Active learning strategies such as laboratory activities can be useful in addressing such misconceptions. In the laboratory exercise presented here, senior undergraduate students explore the intricacies of nonsense suppressor mutations to challenge their understanding of the central dogma. The students introduce a plasmid carrying a nonfunctional chromogenic protein gene due to a nonsense mutation in a codon encoding the chromophore to various nonsense suppressor strains of Escherichia coli . Students then observe distinct chromogenic phenotypes, depending on the suppressor strain. Students showed a moderate increase in understanding of the central dogma. While the central dogma remains a challenging concept, active learning strategies like the one presented here can help reduce conceptual errors.
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来源期刊
Journal of Microbiology & Biology Education
Journal of Microbiology & Biology Education EDUCATION, SCIENTIFIC DISCIPLINES-
CiteScore
3.00
自引率
26.30%
发文量
95
审稿时长
22 weeks
期刊最新文献
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