S. S. Jade, V. N. Gaikwad, S. P. Jadhav, P. S. Takawale, R. A. Bahulikar
{"title":"甲基磺酸乙酯诱变紫花苜蓿M2代的表型变异","authors":"S. S. Jade, V. N. Gaikwad, S. P. Jadhav, P. S. Takawale, R. A. Bahulikar","doi":"10.1071/cp23047","DOIUrl":null,"url":null,"abstract":"Context Mutagenesis is an effective method to create genetic variation in an available accession.Aim This study aimed to generate variability in a genetically distinct genotype of lucerne (Medicago sativa L.) by using a chemical mutagen.Methods Eight different ethyl methanesulfonate (EMS) concentrations were used to determine lethal dose for 50% of the population (LD50). Among them, two EMS concentrations were used to treat 20 000 seeds of BAL-08-01 accession. A total of 8301 individual plants from 1225 families in the second generation (M2) were established under field conditions. Phenotypic observations were recorded as per Distinctness, Uniformity and Stability (DUS) testing guidelines.Key results Wide variations were observed for all phenotypic characters. Among these, green fodder yield, plant height and number of tillers varied with a range of 1–372.5g, 8.1–120.8cm and 0–77 per plant, respectively. Variations were also recorded for other characters such as leaf shape, flower colour, primary branching, etc. A total of 86.76% of plants showed purple colour flowers. Principal component analysis was performed for fodder characters from each EMS concentration. First, two principal components contributed an overall 97.06% variability. M2 plants outside 95% ellipses show the highest phenotypic variations.Conclusion EMS is effective in inducing mutation in lucerne. Phenotypic mutations were recognised in the M2 generation for the quantitative and qualitative characters and green fodder yield.Implications The resulting high level of variability will serve as source material for the improvement of lucerne for various traits.","PeriodicalId":51237,"journal":{"name":"Crop & Pasture Science","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phenotypic variations in M2 generation by ethyl methanesulfonate mutagenesis in lucerne (Medicago sativa L.)\",\"authors\":\"S. S. Jade, V. N. Gaikwad, S. P. Jadhav, P. S. Takawale, R. A. Bahulikar\",\"doi\":\"10.1071/cp23047\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Context Mutagenesis is an effective method to create genetic variation in an available accession.Aim This study aimed to generate variability in a genetically distinct genotype of lucerne (Medicago sativa L.) by using a chemical mutagen.Methods Eight different ethyl methanesulfonate (EMS) concentrations were used to determine lethal dose for 50% of the population (LD50). Among them, two EMS concentrations were used to treat 20 000 seeds of BAL-08-01 accession. A total of 8301 individual plants from 1225 families in the second generation (M2) were established under field conditions. Phenotypic observations were recorded as per Distinctness, Uniformity and Stability (DUS) testing guidelines.Key results Wide variations were observed for all phenotypic characters. Among these, green fodder yield, plant height and number of tillers varied with a range of 1–372.5g, 8.1–120.8cm and 0–77 per plant, respectively. Variations were also recorded for other characters such as leaf shape, flower colour, primary branching, etc. A total of 86.76% of plants showed purple colour flowers. Principal component analysis was performed for fodder characters from each EMS concentration. First, two principal components contributed an overall 97.06% variability. M2 plants outside 95% ellipses show the highest phenotypic variations.Conclusion EMS is effective in inducing mutation in lucerne. Phenotypic mutations were recognised in the M2 generation for the quantitative and qualitative characters and green fodder yield.Implications The resulting high level of variability will serve as source material for the improvement of lucerne for various traits.\",\"PeriodicalId\":51237,\"journal\":{\"name\":\"Crop & Pasture Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Crop & Pasture Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1071/cp23047\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop & Pasture Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1071/cp23047","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Phenotypic variations in M2 generation by ethyl methanesulfonate mutagenesis in lucerne (Medicago sativa L.)
Context Mutagenesis is an effective method to create genetic variation in an available accession.Aim This study aimed to generate variability in a genetically distinct genotype of lucerne (Medicago sativa L.) by using a chemical mutagen.Methods Eight different ethyl methanesulfonate (EMS) concentrations were used to determine lethal dose for 50% of the population (LD50). Among them, two EMS concentrations were used to treat 20 000 seeds of BAL-08-01 accession. A total of 8301 individual plants from 1225 families in the second generation (M2) were established under field conditions. Phenotypic observations were recorded as per Distinctness, Uniformity and Stability (DUS) testing guidelines.Key results Wide variations were observed for all phenotypic characters. Among these, green fodder yield, plant height and number of tillers varied with a range of 1–372.5g, 8.1–120.8cm and 0–77 per plant, respectively. Variations were also recorded for other characters such as leaf shape, flower colour, primary branching, etc. A total of 86.76% of plants showed purple colour flowers. Principal component analysis was performed for fodder characters from each EMS concentration. First, two principal components contributed an overall 97.06% variability. M2 plants outside 95% ellipses show the highest phenotypic variations.Conclusion EMS is effective in inducing mutation in lucerne. Phenotypic mutations were recognised in the M2 generation for the quantitative and qualitative characters and green fodder yield.Implications The resulting high level of variability will serve as source material for the improvement of lucerne for various traits.
期刊介绍:
Crop and Pasture Science (formerly known as Australian Journal of Agricultural Research) is an international journal publishing outcomes of strategic research in crop and pasture sciences and the sustainability of farming systems. The primary focus is broad-scale cereals, grain legumes, oilseeds and pastures. Articles are encouraged that advance understanding in plant-based agricultural systems through the use of well-defined and original aims designed to test a hypothesis, innovative and rigorous experimental design, and strong interpretation. The journal embraces experimental approaches from molecular level to whole systems, and the research must present novel findings and progress the science of agriculture.
Crop and Pasture Science is read by agricultural scientists and plant biologists, industry, administrators, policy-makers, and others with an interest in the challenges and opportunities facing world agricultural production.
Crop and Pasture Science is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.