甲基磺酸乙酯诱变紫花苜蓿M2代的表型变异

IF 1.8 4区 农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY Crop & Pasture Science Pub Date : 2023-01-01 DOI:10.1071/cp23047
S. S. Jade, V. N. Gaikwad, S. P. Jadhav, P. S. Takawale, R. A. Bahulikar
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引用次数: 0

摘要

诱变是一种有效的遗传变异方法。目的利用化学诱变剂对紫花苜蓿(Medicago sativa L.)基因型进行诱变。方法采用8种不同浓度的甲磺酸乙酯(EMS)测定50%种群的致死剂量(LD50)。其中,用2种EMS浓度处理2万株BAL-08-01种子。在田间条件下,共建立了1225科2代(M2)单株8301株。表型观察记录按照独特性,均匀性和稳定性(DUS)测试指南。主要结果:所有表型性状均存在较大差异。其中青饲料产量、株高和分蘖数变化范围分别为1 ~ 372.5g /株、8.1 ~ 120.8cm /株和0 ~ 77个/株。其他性状如叶形、花色、初生分枝等也有变异记录。86.76%的植物呈现紫色花朵。对不同EMS浓度的饲料性状进行主成分分析。首先,两个主成分贡献了97.06%的变异性。95%椭圆外的M2植株表型变异最大。结论EMS是一种有效的诱变方法。在M2代中发现了数量、质量性状和青饲料产量的表型突变。由此产生的高变异水平将为改进苜蓿的各种性状提供来源材料。
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Phenotypic variations in M2 generation by ethyl methanesulfonate mutagenesis in lucerne (Medicago sativa L.)
Context Mutagenesis is an effective method to create genetic variation in an available accession.Aim This study aimed to generate variability in a genetically distinct genotype of lucerne (Medicago sativa L.) by using a chemical mutagen.Methods Eight different ethyl methanesulfonate (EMS) concentrations were used to determine lethal dose for 50% of the population (LD50). Among them, two EMS concentrations were used to treat 20 000 seeds of BAL-08-01 accession. A total of 8301 individual plants from 1225 families in the second generation (M2) were established under field conditions. Phenotypic observations were recorded as per Distinctness, Uniformity and Stability (DUS) testing guidelines.Key results Wide variations were observed for all phenotypic characters. Among these, green fodder yield, plant height and number of tillers varied with a range of 1–372.5g, 8.1–120.8cm and 0–77 per plant, respectively. Variations were also recorded for other characters such as leaf shape, flower colour, primary branching, etc. A total of 86.76% of plants showed purple colour flowers. Principal component analysis was performed for fodder characters from each EMS concentration. First, two principal components contributed an overall 97.06% variability. M2 plants outside 95% ellipses show the highest phenotypic variations.Conclusion EMS is effective in inducing mutation in lucerne. Phenotypic mutations were recognised in the M2 generation for the quantitative and qualitative characters and green fodder yield.Implications The resulting high level of variability will serve as source material for the improvement of lucerne for various traits.
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来源期刊
Crop & Pasture Science
Crop & Pasture Science AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
4.20
自引率
15.80%
发文量
111
审稿时长
3 months
期刊介绍: Crop and Pasture Science (formerly known as Australian Journal of Agricultural Research) is an international journal publishing outcomes of strategic research in crop and pasture sciences and the sustainability of farming systems. The primary focus is broad-scale cereals, grain legumes, oilseeds and pastures. Articles are encouraged that advance understanding in plant-based agricultural systems through the use of well-defined and original aims designed to test a hypothesis, innovative and rigorous experimental design, and strong interpretation. The journal embraces experimental approaches from molecular level to whole systems, and the research must present novel findings and progress the science of agriculture. Crop and Pasture Science is read by agricultural scientists and plant biologists, industry, administrators, policy-makers, and others with an interest in the challenges and opportunities facing world agricultural production. Crop and Pasture Science is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.
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