通过纳米孔测量实现单分子硫醇置换的电化学可视化

IF 4.6 Q1 CHEMISTRY, ANALYTICAL ACS Measurement Science Au Pub Date : 2023-11-09 DOI:10.1021/acsmeasuresciau.3c00046
Chao-Nan Yang, Wei Liu, Hao-Tian Liu, Ji-Chang Zhang, Ru-Jia Yu, Yi-Lun Ying* and Yi-Tao Long, 
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摘要

涉及巯基的反应在维持蛋白质的结构和功能方面起着至关重要的作用。然而,传统的机理研究主要关注的是反应速率和大量溶液中的效率。在此,我们设计了一种半胱氨酸突变纳米孔,作为生物蛋白质纳米反应器,用于硫醇替代反应的电化学可视化。对特征电流信号的统计分析表明,在这种封闭的纳米反应器中,单分子水平的表观反应速率比在大体积溶液中观察到的速率高出 1400 倍。硫醇置换反应在纳米孔内的这种大幅加速为推进微/纳米反应器的设计和优化提供了大有可为的机会。此外,我们的研究结果还可以帮助人们了解生物系统中的巯基反应以及巯基参与的信号转导机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Electrochemical Visualization of Single-Molecule Thiol Substitution with Nanopore Measurement

Reactions involving sulfhydryl groups play a critical role in maintaining the structure and function of proteins. However, traditional mechanistic studies have mainly focused on reaction rates and the efficiency in bulk solutions. Herein, we have designed a cysteine-mutated nanopore as a biological protein nanoreactor for electrochemical visualization of the thiol substitute reaction. Statistical analysis of characteristic current signals shows that the apparent reaction rate at the single-molecule level in this confined nanoreactor reached 1400 times higher than that observed in bulk solution. This substantial acceleration of thiol substitution reactions within the nanopore offers promising opportunities for advancing the design and optimization of micro/nanoreactors. Moreover, our results could shed light on the understanding of sulfhydryl reactions and the thiol-involved signal transduction mechanisms in biological systems.

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来源期刊
ACS Measurement Science Au
ACS Measurement Science Au 化学计量学-
CiteScore
5.20
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0.00%
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0
期刊介绍: ACS Measurement Science Au is an open access journal that publishes experimental computational or theoretical research in all areas of chemical measurement science. Short letters comprehensive articles reviews and perspectives are welcome on topics that report on any phase of analytical operations including sampling measurement and data analysis. This includes:Chemical Reactions and SelectivityChemometrics and Data ProcessingElectrochemistryElemental and Molecular CharacterizationImagingInstrumentationMass SpectrometryMicroscale and Nanoscale systemsOmics (Genomics Proteomics Metabonomics Metabolomics and Bioinformatics)Sensors and Sensing (Biosensors Chemical Sensors Gas Sensors Intracellular Sensors Single-Molecule Sensors Cell Chips Arrays Microfluidic Devices)SeparationsSpectroscopySurface analysisPapers dealing with established methods need to offer a significantly improved original application of the method.
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