塔拉巴州部分植物有机溶剂提取物中植物化学活性成分及对按蚊幼虫的杀虫性能评价

Pukuma M S, Bobbo A A, Qadeer M A, Rufai A
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摘要

合成杀虫剂的持续使用及其毒性问题,加上昆虫抗药性的发生,要求需要替代杀虫剂。植物提取物中含有丰富的生物活性化合物,可作为一种环保杀虫剂的替代来源。研究了塔拉巴州部分植物有机溶剂提取物的植物化学活性成分及对按蚊幼虫的杀灭效果。采用了世界卫生组织的杀虫生物测定规程。将20组4龄按蚊幼虫分别暴露于200,400,600和800ppm的不同浓度下,观察并记录24 h后的死亡率。初步的定性植物化学分析结果显示,一些次生代谢物的存在可能与生物控制电位有关。对第4瞬间按蚊幼虫的全株丙酮提取物的死亡率高于水提取物。200ppm时死亡率最低,为35.0%;800ppm时死亡率最高,为80.0%。LCL -UCL分别为340 ~ 540和722 ~ 1151,Lc50和Lc90值分别为438和866ppm。水提物的生物活性在200ppm时最低,死亡率为36.67%;在800ppm时最高,死亡率为80.0%。LCL -UCL分别为449-806和942-2636,Lc50和Lc90值分别为582和1225ppm。Ancova结果显示,各治疗剂量的平均死亡率差异无统计学意义(p>0.05)。
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Evaluation Of Phytochemical Active Ingredients Present In Organic Solvent Extracts And Larvicidal Properties Of Some Selected Plants From Taraba State Against Anopheles Larvae
The Continuous use of synthetic insecticides and its toxicity problems coupled with the incidence of insect resistance calls for the need for alternative insecticide. Plants extracts a rich source of bioactive compounds can provide an alternative source of insecticide which are ecofriendly. The research evaluated the phytochemical active ingredients present in organic solvent extracts and larvicidal effect of some selected plants from Taraba state against Anopheles larvae. World Health Organisation protocol was adopted for the larvicidal bioassay. Twenty group of fourth instar Anopheles larvae were exposed to various concentrations of 200,400,600 and 800ppm, mortality was observed and recorded after 24 hours. The results of preliminary qualitative phytochemical analyses of tested plants revealed the presence of some secondary metabolite that may be responsible for the bio control potentials. Acetone extract of Hyptis suaveoleons against the fourth instant anopheles larval was observed to have the highest percentage mortality among the entire plants extracts than the aqueous. The lowest mortality was observed at 200ppm with 35.0% while the maximum was at 800ppm with 80.0%. Lc50 and Lc90 values were achieved at 438 and 866ppm with the LCL -UCL 340-540 and 722-1151 respectively. While the biological activity of Hyptis suaveolence aqueous extract had its lowest mortality at 200ppm with 36.67% while the maximum was at 800ppm with 80.0%. Lc50 and Lc90 values were achieved at 582 and 1225ppm with the LCL -UCL 449-806 and 942-2636 respectively. Ancova results showed no significance difference at p>0.05 among the mean percentage mortality of the treated doses.
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