{"title":"磁性微粒固定化酶对尿素转化动力学的聚合物插入效应","authors":"Jin-Won Park","doi":"10.9734/irjpac/2023/v24i5827","DOIUrl":null,"url":null,"abstract":"Urease was covalently immobilized on the magnetic particles without and with polymer between the urease and the particle. The polymer insertion enhanced the conversion from urea to bicarbonate. The ureases were immobilized on the magnetic microparticles through covalent bonds. 150 μL of stock solution of 3 μm diameter particles was washed three times with 20 mM MES rubbing solution at pH 6.0. The results of this study seem to be useful in designing a reactor for recycling wastewater generated in the semiconductor process.","PeriodicalId":14371,"journal":{"name":"International Research Journal of Pure and Applied Chemistry","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Polymer-insertion Effect on Urea Conversion Kinetics via Enzymes Immobilized on Magnetic Microparticles\",\"authors\":\"Jin-Won Park\",\"doi\":\"10.9734/irjpac/2023/v24i5827\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Urease was covalently immobilized on the magnetic particles without and with polymer between the urease and the particle. The polymer insertion enhanced the conversion from urea to bicarbonate. The ureases were immobilized on the magnetic microparticles through covalent bonds. 150 μL of stock solution of 3 μm diameter particles was washed three times with 20 mM MES rubbing solution at pH 6.0. The results of this study seem to be useful in designing a reactor for recycling wastewater generated in the semiconductor process.\",\"PeriodicalId\":14371,\"journal\":{\"name\":\"International Research Journal of Pure and Applied Chemistry\",\"volume\":\"1 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-09-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Research Journal of Pure and Applied Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.9734/irjpac/2023/v24i5827\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Research Journal of Pure and Applied Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9734/irjpac/2023/v24i5827","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
将脲酶共价固定在磁性颗粒上，脲酶与磁性颗粒之间有聚合物和无聚合物。聚合物的加入促进了尿素向碳酸氢盐的转化。这些酶通过共价键固定在磁性微粒上。取粒径为3 μm的原液150 μL，用20 mM MES摩擦液在pH 6.0下洗涤3次。这项研究的结果似乎对设计用于回收半导体过程中产生的废水的反应器有用。
Polymer-insertion Effect on Urea Conversion Kinetics via Enzymes Immobilized on Magnetic Microparticles
Urease was covalently immobilized on the magnetic particles without and with polymer between the urease and the particle. The polymer insertion enhanced the conversion from urea to bicarbonate. The ureases were immobilized on the magnetic microparticles through covalent bonds. 150 μL of stock solution of 3 μm diameter particles was washed three times with 20 mM MES rubbing solution at pH 6.0. The results of this study seem to be useful in designing a reactor for recycling wastewater generated in the semiconductor process.
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