x射线相衬3D虚拟组织学表征结直肠癌复杂的组织结构

Angelika Svetlove, Titus Griebel, Jonas Albers, Lorenzo D’Amico, Philipp Nolte, Giuliana Tromba, Hanibal Bohnenberger, Frauke Alves, Christian Dullin
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引用次数: 0

摘要

肿瘤组织样本的精确形态学分析对于结直肠癌(CRC)的准确诊断和分期至关重要,但仍然受到传统组织学二维性质的限制。我们的目标是通过基于x射线的成像提供组织样本的3D表示,以促进癌症组织中临床相关特征的评估,这一过程目前受到各种限制。在这项研究中,我们发现基于同步辐射传播的自由传播相衬显微计算机断层扫描(SRµCT)适用于使用来自CRC患者的标准福尔马林固定石蜡包埋组织生成2µm体素大小的3D肿瘤体积,并为虚拟组织学提供足够的对比。我们证明,使用现有的配准管道,二维组织学血红素-伊红切片可以放置在3D微CT体积的背景下。然后,精确注册的组织学切片可以用作主要组织学特征的分割和描述的“种子点”。这种方法可以更全面地了解肿瘤在空间中的组织,以及其他结构,如血管、脂肪和淋巴结,并有可能改善患者的预后。
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X-ray phase-contrast 3D virtual histology characterises complex tissue architecture in colorectal cancer
Precise morphological analysis of tumour tissue samples is crucial for accurate diagnosis and staging of colorectal cancer (CRC), but remains limited by the 2D nature of conventional histology. Our aim is to offer a 3D representation of tissue samples by means of X-ray-based imaging to facilitate the evaluation of clinically relevant features in cancer tissue, a process that is currently subject to various restrictions. In this study, we show that propagation-based synchrotron radiation-based free propagation phase-contrast microcomputed tomography (SRµCT) is suitable for the generation of 3D tumour volumes with 2-µm voxel size using standard formalin-fixed, paraffin-embedded tissue from CRC patients and provides sufficient contrast for virtual histology. We demonstrate that, using an existing registration pipeline, a 2D histologic haematoxylin–eosin slice can be placed in the context of the 3D µCT volume. The precisely registered histologic section can then be used as a “seed point” for the segmentation and depiction of major histologic features. This approach allows for a more comprehensive understanding of the organisation of the tumour in space with respect to other structures such as vessels, fat, and lymph nodes, and has the potential to improve patients’ prognostic outcomes.
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