Assessment of tooth brush microbial contamination in dental health care providers: An investigative cross-sectional study

Background: The toothbrush is the most common oral hygiene aid and significantly to reducing oropharyngeal infections. However, it is also the most neglected oral hygiene aid. Objectives: In this cross-sectional investigative study, we assessed the awareness among dental house surgeons regarding their oral hygiene practices, and subsequently their toothbrushes were subjected to microbial assessment. Materials and Methods: A questionnaire regarding the oral hygiene, toothbrush use, and storage was distributed among 96 dental house surgeons. Toothbrushes were collected and the deposit was inoculated in 10 mL of brain heart infusion broth and incubated at 37°C for 24 hours and the growth identified was noted. Results: Of the 77 toothbrush samples that presented with bacterial growth, it was noted that 48% of participants used their toothbrush for 1-3 months. Approximately 82% of participants did not use mouthwash. Although Viridans group of streptococci, a normal commensal of oral cavity was evident, the presence of E. coli, Pseudomonas sp., and Klebsiella sp. was unexpectedly high. Conclusion: Our study has shown that toothbrushes do provide a locale for microorganisms having detrimental effects on general health of the person. Therefore, dental graduates in particular and general population should be educated about maintenance of toothbrushes.