Estimation of Ursolic Acid in Methanolic Extract of Momordica dioica by HPTLC Method

Pharmacognosy Research,2023,15,4,727-732.DOI:10.5530/pres.15.4.077Published:October 2023Type:Original ArticleAuthors:Jui Darbhe, Vrushali Neve, Vrushali Bhalchim, Ashwini Tonpe, Pritam Khandave, Suchita Mane, and Rupali Yewale Author(s) affiliations:Jui Darbhe1, Vrushali Neve1,*, Vrushali Bhalchim1, Ashwini Tonpe1, Pritam Khandave2, Suchita Mane3, Rupali Yewale4 1Department of Pharmacology, Dr. D.Y. Patil Institute of Pharmaceutical Science and Research, Pimpri, Pune, Maharashtra, INDIA. 2Department of Pharmaceutical Chemistry, Sterling Institute of Pharmacy, Nerul, Navi Mumbai, Maharashtra, INDIA. 3Department of Pharmaceutics, Ideal College of Pharmacy, Kalyan, Mumbai, Maharashtra, INDIA. 4Department of Pharmaceutical Chemistry, Konkan Gyanpeeth Rahul Dharkar College of Pharmacy and Research Institute, Karjat, Raigad, Maharashtra, INDIA. Abstract:Background: A number of nonsteroidal anti-inflammatory medications have been shown to lessen pain and inflammation. Unfortunately, there are numerous side effects connected with their use. There are, however, medicinal plants that have anti-inflammatory therapeutic effects with little to no side effects. Momordica dioica is a herb that belongs to the Cucurbitaceae family. There are numerous phytoconstituents in it. Ursolic acid is one of them and a pentacyclic terpenoid that is found in nature and has a number of therapeutic benefits. The current study looked at Ursolic Acid's activities (UA), a secondary plant metabolite, for its anti-inflammatory properties in the Momordica dioica plant. The current study's objective was to create and validate an HPTLC method that was quick, accurate, precise, and specific for determining ursolic acid from Momordica dioica herbal extract. Methodology/Conclusion/Significance: For quick analysis of Ursolic acid determination, The High Performance Thin Layer Chromatography (HPTLC) was established and confirmed. On an aluminium HPTLC plate (60 F254) coated with precoated silica gel with formic acid, ethyl acetate, and toluene (7:3:0,1), chromatographic separation was accomplished. The detection process was carried out at 530 nm. Ursolic acid's Rf value was discovered to be 0.795%. In the 400ng/band concentration range, linearity for ursolic acid was detected. The limits of detection and quantitation for ursolic acid were observed to be 0.04 ng/ band and 0.14 ng/band, respectively. The mean % recovery of rosmarinic acid was (0.54). The method's specificity, robustness, linearity, precision, and accuracy have all been validated in compliance with ICH standards. The created method can be used to evaluate the regularity of ursolic acid in herbal extracts. Keywords:Chromatography, Herbal extract, HPTLC, Inflammation, Methanol, momordica dioica, Rf value, Ursolic acidView:PDF (637.24 KB)