Piper pedicellatum C. DC. Leaf Methanol Extract Inhibits Edema and Granuloma and Modulates Mediators and Cytokines in Rats

Pharmacognosy Research,2023,15,4,679-686.DOI:10.5530/pres.15.4.072Published:October 2023Type:Original ArticleAuthors:Dev Jyoti Kalita, Kangkan Deka, Ngurzampuii Sailo, Sanjoy Das, and Bibhuti Bhusan Kakoti Author(s) affiliations:Dev Jyoti Kalita1,2,*, Kangkan Deka1,3, Ngurzampuii Sailo1, Sanjoy Das1, Bibhuti Bhusan Kakoti1 1Department of Pharmaceutical Sciences, School of Science and Engineering, Dibrugarh University, Dibrugarh, Assam, INDIA. 2Department of Medicine, Assam Medical College and Hospital, Dibrugarh, Assam, INDIA. 3Department of Pharmacognosy, NETES Institute of Pharmaceutical Science, Guwahati. Assam, INDIA. Abstract:Background: Piper pedicellatum is an ethnomedicinal plant used in various inflammatory conditions by many ethnic groups and exploration of its therapeutic potentials is imperative. Objective: This study was carried out to assess anti-inflammatory potential of the methanol extract of the plant leaves using in vivo experiments to validate the traditional uses. Materials and Methods: Carrageenan-induced paw edema and Cotton pellet-induced granuloma models were used to investigate into the anti-inflammatory potential of the plant extract in rats at 200 mg/kg and 400 mg/kg dose. Indomethacin (10 mg/kg) is used as reference standard. Serum levels of inflammatory markers and cytokines, hematological and serum hepatic enzyme indices were analyzed in granuloma-induced model. Results: The methanol extract suppressed Carrageenan-induced paw edema significantly at different time intervals. The highest percentage inhibition was observed at 3rd hr after Carrageenan injection, which was 42.67% and 49.33% for 200 mg/kg and 400 mg/kg dose of the extract respectively. In granuloma-induced model, the methanol extract at 200 mg/kg and 400 mg/kg dose inhibited exudate formation by 28.58% and 41.27%, respectively and inhibition of granuloma tissue was 32.45% and 43.71%, respectively along with significant decrement in serum C-reactive protein, Prostaglandin E2, Tumor necrosis factor-α, Interleukin-6 while increases in Interleukin-10 levels. Also, the results showed hematological and serum hepatic enzyme levels were rectified to near normal levels in treated rats. Conclusion: The outcome of the investigation reveals the potential of extract as possible anti-inflammatory agent, which can be develop into therapeutic agent or supplement and also necessitates further pharmacological studies on the plant. Keywords:Cytokines, Edema, Granuloma, Interleukins, Piper pedicellatum C. DCView:PDF (693.95 KB)