多种内生芽孢杆菌改善突尼斯马铃薯土传和结核传疾病的控制:从实验室到田间条件

IF 2.1 3区 农林科学 Q1 ENTOMOLOGY Egyptian Journal of Biological Pest Control Pub Date : 2023-11-07 DOI:10.1186/s41938-023-00753-5
Hayfa Jabnoun-Khiareddine, Rania Aydi-Ben-Abdallah, Mejda Daami-Remadi
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引用次数: 0

摘要

摘要背景枯萎病、根核菌溃疡病和黑点病由于其病原菌的土传性和结核传性,在突尼斯和世界范围内仍是马铃薯生产中的严重问题。在内生细菌中,芽孢杆菌属是开发最多的微生物群之一,被称为对几种马铃薯疾病的有效生物防治剂。本研究对5个芽孢杆菌种的6株芽孢杆菌进行了体外、体内和自然条件下3个种植季对马铃薯根系和枯萎病菌的抑制能力和促进植株生长的能力的筛选。结果双培养实验表明,SV39、SV104(龙基芽孢杆菌)、SV41(枯草芽孢杆菌)、SV44(甲基营养性芽孢杆菌)和SV65(解淀粉芽孢杆菌亚种)的全细胞悬液均具有良好的分离活性。植物芽孢杆菌(Plantarum)菌株对重要的马铃薯土传植物病原菌具有较强的抗真菌活性,抑制率为65% ~ 70%。无细胞培养滤液、丁醇和氯甲酸提取物对细菌的抑制率也显著,这取决于目标病原体、使用的浓度和所测试的芽孢杆菌菌株。盆栽试验中,所有芽孢杆菌菌株处理均可使根核菌的溃疡严重程度较接种对照和未处理对照降低43% ~ 65%,其中SV39、S42和sv65处理的黑点严重程度和相对维管变色程度分别较阳性对照降低50% ~ 53%和65 ~ 52%。这种生物防治潜力与马铃薯生长参数的提高有关。田间研究表明,用大多数芽孢杆菌菌株处理土壤能显著控制所有目标真菌土传病害,并至少改善两个生长和/或生产参数,具体取决于使用的菌株和种植季节。以SV39-、SV41-、SV44-和sv104为基础的处理可显著提高块茎单季产量。结论在马铃薯有机或常规生产体系下,这些芽孢杆菌可与其他现有方法组合或引进,以补充控制目标病害和提高产量。
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Multi-species endophytic Bacillus for improved control of potato soilborne and tuber-borne diseases in Tunisia: from laboratory to field conditions
Abstract Background Due to the soilborne and tuber-borne nature of their causative agents, Fusarium wilt, Rhizoctonia root canker and black dot diseases are still leading to serious problems in potato production in Tunisia and worldwide. Among endophytic bacteria, the genus Bacillus is one of the most exploited microbial groups known as potent biocontrol agents against several potato diseases. In the present study, six strains belonging to five Bacillus species were screened for their abilities to inhibit root and wilt potato pathogens in vitro, in vivo and under natural conditions over three cropping seasons and to promote plant growth. Results Based on the dual-culture assays, the whole-cell suspensions of SV39 and SV104 ( Bacillus tequilensis ), SV41 ( B. subtilis ), SV44 ( B. methylotrophicus ) and SV65 ( B. amyloliquefaciens subsp. plantarum ) strains exhibited potent antifungal activity against important potato soilborne phytopathogens with ~ 65 to 70% inhibition rates. Significant inhibition rates were also induced by the cell-free culture filtrates, the butanolic and the chloroformic extracts depending on the target pathogens, the concentration used and the Bacillus strain tested. In pot experiment, a decrease in Rhizoctonia root canker severity, ranging from 43 to 65% compared to the inoculated and untreated control, was induced by all Bacillus spp. strain-based treatments, while SV39-, S42- and SV65-based treatments were the most effective in suppressing by 50–53 and 65–52%, black dot severity and the relative vascular discoloration extent induced by F. oxysporum f. sp. tuberosi , respectively, relative to positive control. This biocontrol potential was associated with an enhancement of potato growth parameters. Field studies indicated that soil treatment with the most of the Bacillus spp. strains had significantly controlled all the target fungal soilborne diseases and improved at the least two growth and/or production parameters depending on the strain used and the cropping seasons. SV39-, SV41-, SV44- and SV104-based treatments resulted in a significant increase in tuber yield in one cropping season. Conclusion These Bacillus spp. strains could be used in combinations and/or introduced with other existing practices in order to provide supplemental control of target diseases and yield promotion under organic or conventional potato production systems.
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来源期刊
CiteScore
4.90
自引率
16.70%
发文量
128
审稿时长
>12 weeks
期刊介绍: The Egyptian Journal of Biological Pest Control is a periodic scientific journal published by the Egyptian Society for Biological Control of Pests (ESBCP) in collaboration with SpringerNature. The journal aims to publish internationally peer-reviewed, high-quality research articles in the field of biological and integrated pest control (non-chemical control). The journal publishes review articles, original papers, conference reports, book reviews, editorials, laboratory reports, technical notes and short communications.
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