球孢白僵菌在不同培养基上的推广及其对昆虫的致病性

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摘要

球孢白僵菌(Beauveria bassiana)是一种已知能有效控制重要植物害虫的昆虫病原真菌。球孢白僵菌(b.s bassiana)具有较高的繁殖能力,在自然界中容易产生,在不利条件下也能形成持续时间较长的孢子。本研究的目的是测定球孢白僵菌孢子在玉米和水稻培养基上的密度和活力,并探讨球孢白僵菌侵染试验昆虫的过程。所使用的方法是;真菌在水稻和玉米培养基上的繁殖试验,玉米和水稻培养基上真菌的显微观察,真菌孢子密度计算,真菌活力计算和致病性试验。研究结果如下:结果球孢白僵菌在第7天开始繁殖,真菌在水稻培养基中的生长速度快于在玉米培养基中的生长速度。球孢黑僵菌在玉米培养基上的孢子密度为3.75 × 108孢子/ml,水稻培养基上的孢子密度为4.58 × 108孢子/ml,咖啡培养基上的孢子密度为3.5 × 108孢子/ml,水稻培养基上的孢子密度为3.75 × 108孢子/ml。球孢白僵菌、丁香白僵菌和banyuwangi咖啡在水稻和玉米培养基上的存活率平均在60%以上。球孢白僵菌对昆虫的致病性试验结果在投药后第10天开始出现。
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Promotion of Beauveria bassiana Mushroom on Different Growing Media and Its Pathogenicity on Insects
One type of entomopathogenic fungus that is known to be effective in controlling important plant pests is the Beauveria bassiana fungus. The fungus B. bassiana, has a high reproductive capacity, is easy to produce and under unfavorable conditions can form spores that can last a long time in nature. The purpose of this study was to determine the density and viability of B. bassiana spores on corn and rice media and to investigate the process of B. bassiana fungal infection in the test insects. The method used is; Fungal propagation test on Rice and Corn Media, Microscopic Observation of Fungus from Corn and Rice Media, Calculation of Fungus Spore Density, Calculation of Fungus Viability and Pathogenicity Test. The research results obtained are as follows; Results The propagation of B. bassiana mushroom grew on the seventh day, the fungus growth was faster in rice media than in corn media. The spore density of the fungus B. bassiana clove bondowoso in corn media was 3.75x108 spores/ml, rice media was 4.58x108 spores/ml, B. bassiana banyuwangi coffee medium was 3.5x108 spores/ml, rice media was 3.75x108 spores/ml. The viability of the fungus B. bassiana, clove bondowoso and banyuwangi coffee on rice and corn media was obtained on average above 60%. The results of the pathogenicity test of B. bassiana on insects began to appear on the tenth day after the baiting process.
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