活动硬骨鱼布鲁克鳟鱼(Salvelinus fontinalis)全身和红肌线粒体呼吸的比较

IF 1 4区 生物学 Q3 ZOOLOGY Canadian Journal of Zoology Pub Date : 2023-09-27 DOI:10.1139/cjz-2023-0045
Travis Durhack, Melanie Aminot, Jason Treberg, Eva Enders
{"title":"活动硬骨鱼布鲁克鳟鱼(Salvelinus fontinalis)全身和红肌线粒体呼吸的比较","authors":"Travis Durhack, Melanie Aminot, Jason Treberg, Eva Enders","doi":"10.1139/cjz-2023-0045","DOIUrl":null,"url":null,"abstract":"Understanding how metabolic costs change in relation to increasing temperature under future climate changes is important to predict how ectotherms will be affected across the globe. In fish, whole body respiration is traditionally used to estimate aerobic performance via an organism’s minimum and maximum oxygen consumption rates. However, mitochondria play a crucial role in the aerobic cascade and may be a useful surrogate of aerobic performance. To test whether whole body oxygen consumption and mitochondrial capacity are correlated, we estimated whole body metabolic and mitochondrial respiration rates (using permeabilized red muscle fibres) in brook trout ( Salvelinus fontinalis (Mitchill, 1814)) at 10, 15, and 20 °C. Standard metabolic rate increased with acclimation temperature, while maximum rates were less sensitive. All mitochondrial respiration rates increased with acclimation temperature, suggesting that red muscle mitochondrial preparations may correlate to the minimal metabolic demands in this species. When expressed as relative rates of electron flow, the red muscle fibres showed no effect of temperature on mitochondrial coupling efficiency. However, there was a pattern of declining capacity to augment respiration via complex II with increasing temperature with a concomitant increase in the capacity of the phosphorylating system relative to maximal rates of mitochondrial electron flow.","PeriodicalId":9484,"journal":{"name":"Canadian Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparing whole body and red muscle mitochondrial respiration in an active teleost fish, Brook Trout (Salvelinus fontinalis)\",\"authors\":\"Travis Durhack, Melanie Aminot, Jason Treberg, Eva Enders\",\"doi\":\"10.1139/cjz-2023-0045\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Understanding how metabolic costs change in relation to increasing temperature under future climate changes is important to predict how ectotherms will be affected across the globe. In fish, whole body respiration is traditionally used to estimate aerobic performance via an organism’s minimum and maximum oxygen consumption rates. However, mitochondria play a crucial role in the aerobic cascade and may be a useful surrogate of aerobic performance. To test whether whole body oxygen consumption and mitochondrial capacity are correlated, we estimated whole body metabolic and mitochondrial respiration rates (using permeabilized red muscle fibres) in brook trout ( Salvelinus fontinalis (Mitchill, 1814)) at 10, 15, and 20 °C. Standard metabolic rate increased with acclimation temperature, while maximum rates were less sensitive. All mitochondrial respiration rates increased with acclimation temperature, suggesting that red muscle mitochondrial preparations may correlate to the minimal metabolic demands in this species. When expressed as relative rates of electron flow, the red muscle fibres showed no effect of temperature on mitochondrial coupling efficiency. However, there was a pattern of declining capacity to augment respiration via complex II with increasing temperature with a concomitant increase in the capacity of the phosphorylating system relative to maximal rates of mitochondrial electron flow.\",\"PeriodicalId\":9484,\"journal\":{\"name\":\"Canadian Journal of Zoology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2023-09-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Canadian Journal of Zoology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1139/cjz-2023-0045\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Canadian Journal of Zoology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1139/cjz-2023-0045","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ZOOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

了解在未来气候变化下,代谢成本如何随温度升高而变化,对于预测全球变温动物将如何受到影响非常重要。在鱼类中,全身呼吸通常通过生物体的最小和最大耗氧量来估计有氧性能。然而,线粒体在有氧级联中起着至关重要的作用,可能是有氧性能的有用替代品。为了测试全身耗氧量和线粒体能力是否相关,我们在10、15和20°C时估算了溪鳟(Salvelinus fontinalis (Mitchill, 1814))的全身代谢和线粒体呼吸速率(使用渗透红色肌纤维)。标准代谢率随驯化温度升高而升高,而最大代谢率对驯化温度不敏感。所有线粒体呼吸速率都随着驯化温度的升高而增加,这表明红肌线粒体制剂可能与该物种的最低代谢需求有关。当以相对电子流速率表示时,红肌纤维显示温度对线粒体偶联效率没有影响。然而,随着温度的升高,通过复合体II增强呼吸的能力下降,同时磷酸化系统的能力相对于线粒体电子流的最大速率增加。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Comparing whole body and red muscle mitochondrial respiration in an active teleost fish, Brook Trout (Salvelinus fontinalis)
Understanding how metabolic costs change in relation to increasing temperature under future climate changes is important to predict how ectotherms will be affected across the globe. In fish, whole body respiration is traditionally used to estimate aerobic performance via an organism’s minimum and maximum oxygen consumption rates. However, mitochondria play a crucial role in the aerobic cascade and may be a useful surrogate of aerobic performance. To test whether whole body oxygen consumption and mitochondrial capacity are correlated, we estimated whole body metabolic and mitochondrial respiration rates (using permeabilized red muscle fibres) in brook trout ( Salvelinus fontinalis (Mitchill, 1814)) at 10, 15, and 20 °C. Standard metabolic rate increased with acclimation temperature, while maximum rates were less sensitive. All mitochondrial respiration rates increased with acclimation temperature, suggesting that red muscle mitochondrial preparations may correlate to the minimal metabolic demands in this species. When expressed as relative rates of electron flow, the red muscle fibres showed no effect of temperature on mitochondrial coupling efficiency. However, there was a pattern of declining capacity to augment respiration via complex II with increasing temperature with a concomitant increase in the capacity of the phosphorylating system relative to maximal rates of mitochondrial electron flow.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Canadian Journal of Zoology
Canadian Journal of Zoology 生物-动物学
CiteScore
2.40
自引率
0.00%
发文量
82
审稿时长
3 months
期刊介绍: Published since 1929, the Canadian Journal of Zoology is a monthly journal that reports on primary research contributed by respected international scientists in the broad field of zoology, including behaviour, biochemistry and physiology, developmental biology, ecology, genetics, morphology and ultrastructure, parasitology and pathology, and systematics and evolution. It also invites experts to submit review articles on topics of current interest.
期刊最新文献
Spatiotemporal distribution of the non-indigenous peach blossom jellyfish Craspedacusta sowerbii in British Columbia, Canada Spatiotemporal distribution of the non-indigenous peach blossom jellyfish Craspedacusta sowerbii in British Columbia, Canada Nursing behavior of wild polar bears in the Canadian High Arctic Seasonal dynamics of small mammal populations: resource availability and cold exposure interact to govern abundance Determinants of Multiple Brooding in Barn Swallows (Hirundo rustica) in Atlantic Canada
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1