Enteric virus removal by municipal wastewater treatment to achieve requirements for potable reuse

Primary and secondary treatment of municipal wastewater contributes to virus removal upstream of advanced purification to produce water for potable reuse. In this study, virus occurrence by cultivable and molecular methods was measured over a 24-month period in raw wastewater influents and secondary effluents from two municipal wastewater treatment plants that together provide the recycled water source for an advanced water purification facility. Using a rank-paired, covariance-based statistical approach, virus log removal values were determined for four wastewater treatment processes that operate in parallel at the two facilities (two activated sludge processes, trickling filter process, and trickling filter/solids contactor process). The trickling filter process exhibited the lowest observed removal of cultivable enteric virus with a median removal of 1.0 log 10 (or 90% removal) and a 5 th percentile log removal of 0.73 (or 82%), compared to the greatest removal observed for one of the activated sludge processes (median log removal of 2.4 or 99.6% and 5 th percentile of 2.1 or 99.2%). Median log removal observed for male-specific (MS) and somatic (SOM) coliphage was 1.8 (98.6% removal) and 0.5 (70%), respectively, for trickling filter and 2.9 (99.9%) and 2.0 (99%) for activated sludge. Thus, coliphage removal was fairly similar to removal observed for cultivable enteric virus. The cultivable enteric virus 5 th percentile removal (0.7) from the trickling filter treatment process was proposed to the state regulator for credit towards state requirements for virus removal related to groundwater augmentation with purified recycled water. Receiving pathogen removal credits for secondary wastewater treatment would allow for an improved margin (safety factor) of credits beyond the minimum required; and in this case may also increase the number of viable future groundwater recharge sites closer to drinking water production wells by reducing the underground travel time otherwise required to obtain sufficient credits.