Designing a bacterial cellulose‐based hydrogel incorporated with manganese sulfide and graphene oxide for green extraction of acrylamide in bread samples

Abstract A novel nanocomposite based on bacterial cellulose (BC) modified by manganese sulfide (MnS) decorated graphene oxide (GO) was prepared. The hydrogel was characterized by Fourier transform infrared, scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, and adsorption/desorption techniques. The sorbent was employed for determining acrylamide in bread samples using micro‐solid‐phase extraction coupled with high‐performance liquid chromatography. The extraction efficiency of prepared sorbent was 1.4‐fold higher than that of BC. The important extraction factors including elution conditions (acetonitrile 300 μL, 5 min), sorbent composition and mass (50 mg), adsorption time (7 min), sample solution pH (8), and salt effect (3% w/v) were considered for optimization of the method. The analytical merit was validated by assessing the preconcentration factor (178–225), limit of detection (1.56 μg/kg), limit of quantification (5.15 μg/kg), linearity (5.15–500 μg/kg), and determination coefficient (0.9845). The adsorption isotherms and kinetic studies demonstrated that the adsorption process followed the Langmuir model and pseudo‐second‐order kinetics. The relative recoveries and relative standard deviations were 84%–106% and 2.4%–9.2%, respectively. This paper is the first report on the fabrication and use of BC doped with MnS‐GO composite for extraction of acrylamide in typical commercial bread samples.