{"title":"芳香植物精油中的茶叶烯抗高尿酸血症的潜力：基于 SiO2-AgNO3 的柱层析纯化、抗氧化和黄嘌呤氧化酶抑制活性","authors":"Arif Setiawansyah, Muh Ikhlas Arsul, Sukrasno Sukrasno, Sophi Damayanti, Muhamad Insanu, Irda Fidrianny","doi":"10.1007/s13596-023-00710-5","DOIUrl":null,"url":null,"abstract":"<div><p>Xanthine oxidase is an enzyme that plays an essential role in the biosynthesis of uric acid. Inhibiting this enzyme can diminish the production of uric acid. Caryophyllene is a type 2 cannabinoid agonist receptor widely distributed in all plants' essential oil and provides plentiful biological activities. This research was carried out to isolate and evaluate the antioxidant and xanthine oxidase inhibitory activities of caryophyllene. Caryophyllene was isolated from <i>Syzygium aromaticum</i> essential oil using SiO<sub>2</sub>-AgNO<sub>3</sub>-based column chromatography. The xanthine oxidase inhibitory activity was assessed by molecular docking and in vitro assay using a spectrophotometer in 96-well plates below the aerobic conditions, while the antioxidant activity was tested to DPPH and measured as AAI. Our study revealed that the SiO<sub>2</sub>-AgNO<sub>3</sub>-based column chromatography technique provides caryophyllene with a purity of 98%. The biological evaluation showed that caryophyllene could inhibit the xanthine oxidase in silico and in vitro with an IC<sub>50</sub> value of 4.79 ± 0.25 µg/mL. Caryophyllene also exhibits very strong antioxidant activity. In conclusion, caryophyllene shows a promising potential to be developed as a novel candidate for uric acid-lowering agents.</p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 2","pages":"475 - 487"},"PeriodicalIF":1.8000,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Anti-hyperuricemic potential of caryophyllene from Syzygium aromaticum essential oil: SiO2-AgNO3-based column chromatography purification, antioxidant, and xanthine oxidase inhibitory activities\",\"authors\":\"Arif Setiawansyah, Muh Ikhlas Arsul, Sukrasno Sukrasno, Sophi Damayanti, Muhamad Insanu, Irda Fidrianny\",\"doi\":\"10.1007/s13596-023-00710-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Xanthine oxidase is an enzyme that plays an essential role in the biosynthesis of uric acid. Inhibiting this enzyme can diminish the production of uric acid. Caryophyllene is a type 2 cannabinoid agonist receptor widely distributed in all plants' essential oil and provides plentiful biological activities. This research was carried out to isolate and evaluate the antioxidant and xanthine oxidase inhibitory activities of caryophyllene. Caryophyllene was isolated from <i>Syzygium aromaticum</i> essential oil using SiO<sub>2</sub>-AgNO<sub>3</sub>-based column chromatography. The xanthine oxidase inhibitory activity was assessed by molecular docking and in vitro assay using a spectrophotometer in 96-well plates below the aerobic conditions, while the antioxidant activity was tested to DPPH and measured as AAI. Our study revealed that the SiO<sub>2</sub>-AgNO<sub>3</sub>-based column chromatography technique provides caryophyllene with a purity of 98%. The biological evaluation showed that caryophyllene could inhibit the xanthine oxidase in silico and in vitro with an IC<sub>50</sub> value of 4.79 ± 0.25 µg/mL. Caryophyllene also exhibits very strong antioxidant activity. In conclusion, caryophyllene shows a promising potential to be developed as a novel candidate for uric acid-lowering agents.</p><h3>Graphical abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":7613,\"journal\":{\"name\":\"Advances in Traditional Medicine\",\"volume\":\"24 2\",\"pages\":\"475 - 487\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2023-09-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advances in Traditional Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13596-023-00710-5\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in Traditional Medicine","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1007/s13596-023-00710-5","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
Anti-hyperuricemic potential of caryophyllene from Syzygium aromaticum essential oil: SiO2-AgNO3-based column chromatography purification, antioxidant, and xanthine oxidase inhibitory activities
Xanthine oxidase is an enzyme that plays an essential role in the biosynthesis of uric acid. Inhibiting this enzyme can diminish the production of uric acid. Caryophyllene is a type 2 cannabinoid agonist receptor widely distributed in all plants' essential oil and provides plentiful biological activities. This research was carried out to isolate and evaluate the antioxidant and xanthine oxidase inhibitory activities of caryophyllene. Caryophyllene was isolated from Syzygium aromaticum essential oil using SiO2-AgNO3-based column chromatography. The xanthine oxidase inhibitory activity was assessed by molecular docking and in vitro assay using a spectrophotometer in 96-well plates below the aerobic conditions, while the antioxidant activity was tested to DPPH and measured as AAI. Our study revealed that the SiO2-AgNO3-based column chromatography technique provides caryophyllene with a purity of 98%. The biological evaluation showed that caryophyllene could inhibit the xanthine oxidase in silico and in vitro with an IC50 value of 4.79 ± 0.25 µg/mL. Caryophyllene also exhibits very strong antioxidant activity. In conclusion, caryophyllene shows a promising potential to be developed as a novel candidate for uric acid-lowering agents.
期刊介绍：
Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.
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