探讨甲烷氧化菌促进水稻作物生长的潜力

Methane Pub Date : 2023-09-27 DOI:10.3390/methane2040024
Jyoti A. Mohite, Kumal Khatri, Kajal Pardhi, Shubha S. Manvi, Rutuja Jadhav, Shilpa Rathod, Monali C. Rahalkar
{"title":"探讨甲烷氧化菌促进水稻作物生长的潜力","authors":"Jyoti A. Mohite, Kumal Khatri, Kajal Pardhi, Shubha S. Manvi, Rutuja Jadhav, Shilpa Rathod, Monali C. Rahalkar","doi":"10.3390/methane2040024","DOIUrl":null,"url":null,"abstract":"Rice fields are one of the important anthropogenic sources of methane emissions. Methanotrophs dwelling near the rice roots and at the oxic–anoxic interface of paddy fields can oxidize a large fraction of the generated methane and are therefore considered to be important. Nitrogen fixation in rice root-associated methanotrophs is well known. Our aim in this study was to explore the potential of methanotrophs as bio-inoculants for rice and the studies were performed in pot experiments in monsoon. Ten indigenously isolated methanotrophs were used belonging to eight diverse genera of Type Ia, Type Ib, and Type II methanotrophs, including the newly described genera and/or species, Methylocucumis oryzae and Methylolobus aquaticus, as well as Ca. Methylobacter oryzae and Ca. Methylobacter coli. Additionally, two consortia (Methylomonas strains and Methylocystis-Methylosinus strains) were used. Nitrogen fixation pathways or nifH genes were detected in all of the used methanotrophs. Plant growth promotion (PGPR) was seen in terms of increased plant height and grain yield. Nine out of twelve (seven single strains and two consortia) showed positive effects on grain yield (6–38%). The highest increase in grain yield was seen after inoculation with Ca. Methylobacter coli (38%) followed by Methylomonas consortium (35%) and Methylocucumis oryzae (31%). Methylomagnum ishizawai inoculated plants showed the highest plant height. Methylocucumis oryzae inoculated plants showed early flowering, grain formation, and grain maturation (~17–18 days earlier). In all the pot experiments, minimal quantities of nitrogen fertilizer were used with no additional organic fertilizer inputs. The present study demonstrated the possibility of developing methanotrophs as bio-inoculants for rice agriculture, which would promote plant growth under low inputs of nitrogenous fertilizers. Although the effect of methanotrophs on methane mitigation is still under investigation, their application to reduce methane emissions from rice fields could be an added advantage.","PeriodicalId":74177,"journal":{"name":"Methane","volume":"150 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exploring the Potential of Methanotrophs for Plant Growth Promotion in Rice Agriculture\",\"authors\":\"Jyoti A. Mohite, Kumal Khatri, Kajal Pardhi, Shubha S. Manvi, Rutuja Jadhav, Shilpa Rathod, Monali C. Rahalkar\",\"doi\":\"10.3390/methane2040024\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Rice fields are one of the important anthropogenic sources of methane emissions. Methanotrophs dwelling near the rice roots and at the oxic–anoxic interface of paddy fields can oxidize a large fraction of the generated methane and are therefore considered to be important. Nitrogen fixation in rice root-associated methanotrophs is well known. Our aim in this study was to explore the potential of methanotrophs as bio-inoculants for rice and the studies were performed in pot experiments in monsoon. Ten indigenously isolated methanotrophs were used belonging to eight diverse genera of Type Ia, Type Ib, and Type II methanotrophs, including the newly described genera and/or species, Methylocucumis oryzae and Methylolobus aquaticus, as well as Ca. Methylobacter oryzae and Ca. Methylobacter coli. Additionally, two consortia (Methylomonas strains and Methylocystis-Methylosinus strains) were used. Nitrogen fixation pathways or nifH genes were detected in all of the used methanotrophs. Plant growth promotion (PGPR) was seen in terms of increased plant height and grain yield. Nine out of twelve (seven single strains and two consortia) showed positive effects on grain yield (6–38%). The highest increase in grain yield was seen after inoculation with Ca. Methylobacter coli (38%) followed by Methylomonas consortium (35%) and Methylocucumis oryzae (31%). Methylomagnum ishizawai inoculated plants showed the highest plant height. Methylocucumis oryzae inoculated plants showed early flowering, grain formation, and grain maturation (~17–18 days earlier). In all the pot experiments, minimal quantities of nitrogen fertilizer were used with no additional organic fertilizer inputs. The present study demonstrated the possibility of developing methanotrophs as bio-inoculants for rice agriculture, which would promote plant growth under low inputs of nitrogenous fertilizers. Although the effect of methanotrophs on methane mitigation is still under investigation, their application to reduce methane emissions from rice fields could be an added advantage.\",\"PeriodicalId\":74177,\"journal\":{\"name\":\"Methane\",\"volume\":\"150 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-09-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Methane\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/methane2040024\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methane","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/methane2040024","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

稻田是甲烷排放的重要人为来源之一。居住在水稻根系附近和稻田缺氧-缺氧界面的甲烷氧化菌可以氧化大部分产生的甲烷,因此被认为是重要的。水稻根相关甲烷氧化菌的固氮作用是众所周知的。本研究的目的是探索甲烷氧化菌作为水稻生物接种剂的潜力,并在季风季节进行了盆栽试验。利用10个本地分离的甲烷养菌,分别属于Ia型、Ib型和II型甲烷养菌的8个不同属,包括新发现的属和/或种,稻谷甲基化菌和水生甲基化菌,以及稻谷甲基化菌和大肠甲基化菌。此外,还使用了两个菌株(甲基单胞菌菌株和甲基胞菌-甲基窦菌菌株)。在所有使用过的甲烷氧化菌中均检测到固氮途径或nifH基因。植物生长促进(PGPR)表现在株高和籽粒产量的增加。12个菌株中有9个(7个单株和2个菌落)对籽粒产量有正向影响(6-38%)。以接种甲基大肠杆菌(Ca. Methylobacter coli)增产幅度最大(38%),其次是联合甲基单胞菌(Methylomonas consortium)(35%)和米紫甲基单胞菌(methylocumis oryzae)(31%)。接种石崎甲麻植株株高最高。稻瘟病菌接种植株开花、籽粒形成和成熟均提前17 ~ 18 d。在所有盆栽试验中,施用少量氮肥,不添加有机肥。研究结果表明,在低氮肥投入条件下,开发甲烷氧化菌作为水稻生物接种剂具有促进水稻生长的潜力。虽然甲烷氧化菌对甲烷减排的影响仍在调查中,但将其用于减少稻田的甲烷排放可能是一个额外的优势。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Exploring the Potential of Methanotrophs for Plant Growth Promotion in Rice Agriculture
Rice fields are one of the important anthropogenic sources of methane emissions. Methanotrophs dwelling near the rice roots and at the oxic–anoxic interface of paddy fields can oxidize a large fraction of the generated methane and are therefore considered to be important. Nitrogen fixation in rice root-associated methanotrophs is well known. Our aim in this study was to explore the potential of methanotrophs as bio-inoculants for rice and the studies were performed in pot experiments in monsoon. Ten indigenously isolated methanotrophs were used belonging to eight diverse genera of Type Ia, Type Ib, and Type II methanotrophs, including the newly described genera and/or species, Methylocucumis oryzae and Methylolobus aquaticus, as well as Ca. Methylobacter oryzae and Ca. Methylobacter coli. Additionally, two consortia (Methylomonas strains and Methylocystis-Methylosinus strains) were used. Nitrogen fixation pathways or nifH genes were detected in all of the used methanotrophs. Plant growth promotion (PGPR) was seen in terms of increased plant height and grain yield. Nine out of twelve (seven single strains and two consortia) showed positive effects on grain yield (6–38%). The highest increase in grain yield was seen after inoculation with Ca. Methylobacter coli (38%) followed by Methylomonas consortium (35%) and Methylocucumis oryzae (31%). Methylomagnum ishizawai inoculated plants showed the highest plant height. Methylocucumis oryzae inoculated plants showed early flowering, grain formation, and grain maturation (~17–18 days earlier). In all the pot experiments, minimal quantities of nitrogen fertilizer were used with no additional organic fertilizer inputs. The present study demonstrated the possibility of developing methanotrophs as bio-inoculants for rice agriculture, which would promote plant growth under low inputs of nitrogenous fertilizers. Although the effect of methanotrophs on methane mitigation is still under investigation, their application to reduce methane emissions from rice fields could be an added advantage.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Application of Digestate from a Methane Fermentation Process for Supplying Water and Nutrients in Sweet Potato Cultivation in Sandy Soil Pathways toward Climate-Neutral Red Meat Production Recent Advances in the Use of Controlled Nanocatalysts in Methane Conversion Reactions Dry Reforming of CH4 Using a Microreactor A Study on the Heterogeneity and Anisotropy of the Porous Grout Body Created in the Stabilization of a Methane Hydrate Reservoir through Grouting
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1