黑松露块菌离体培养条件的优化

IF 0.9 4区 生物学 Q4 PLANT SCIENCES Botanica Serbica Pub Date : 2023-01-01 DOI:10.2298/botserb2302259s
Reza Salehi Molkabadi, Gregory Bonito, Kamran Ghasemi, Mohammad Ghanbary, Fatemeh Raouf Fard
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引用次数: 0

摘要

外生菌根松露真菌的无性繁殖受到其菌丝生长缓慢的限制。包括培养基、分离物基因型和环境条件在内的许多因素都可以改变真菌菌丝的生长速度。本研究旨在通过确定最佳碳水化合物和氮源、温度和ph来提高棕块茎的体外生长速度。在以葡萄糖为主要碳水化合物的培养基中,8周后棕块茎的生长水平最高,菌丝分枝密度最大。对于氮,与其他氨基酸处理相比,谷氨酰胺(200 mg N -1)提供了最大的菌丝生长和密度。关于温度,16?C被证明是最适合棕毛霉生长和分枝的。pH为6和pH为7的培养基最有利于褐条霉的生长。本研究结果为黑松露菌丝的体外培养提供了基础数据,并对冬松露菌丝的体外培养具有指导意义。
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Optimising in vitro culture conditions for the truffle Tuber brumale
The vegetative propagation of ectomycorrhizal truffle fungi is limited by their slow mycelial growth. Many factors including media, isolate genotypes and environmental conditions can alter fungal mycelial growth rates. This study aimed to improve the in vitro growth rate of Tuber brumale by determining the optimal carbohydrate and nitrogen sources, temperature and pH. After 8 weeks, the highest level of growth and densest hyphal branching were recorded in the medium containing glucose as the main carbohydrate. For nitrogen, glutamine (200 mg N l-1) provided the greatest hyphal growth and density compared to the other amino acid treatments. Regarding temperature, 16?C proved to be optimal for T. brumale growth and branching. Media of pH 6 and pH 7 were most favourable for the growth of T. brumale. The results from this research provide baseline data on the vegetative nutrition of T. brumale and have implications for the in vitro culture of winter truffle hyphae.
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来源期刊
Botanica Serbica
Botanica Serbica Agricultural and Biological Sciences-Plant Science
CiteScore
1.40
自引率
12.50%
发文量
17
审稿时长
34 weeks
期刊介绍: Botanica Serbica publishes original research papers on all aspects of plant, fungal and microbial biology research including the disciplines of microbiology, mycology, lichenology, bryology, flora, vegetation, biogeography, systematics, taxonomy, plant biotechnology, plant cell biology, plant ecology, environmental plant biology, forestry, genomics, horticulture, limnology, metabolomics, molecular biology, proteomics, virology, plant conservation and protection, and wildlife and ecosystem management.
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