{"title":"激发子提高了双头金丝桃茎部培养物中生物活性化合物的产生","authors":"Hilal Surmuş Asan","doi":"10.2298/botserb2302271s","DOIUrl":null,"url":null,"abstract":"In this study, we examined the effects of elicitors MeJA (Methyl jasmonate) and JA (Jasmonic acid) (50, 100, and 200 ?M) on the growth and production of 15 phenolic compounds in shoot cultures of Hypericum amblysepalum using the LC-MS/MS method. The biomass production increased on shoots elicited with 50 ?M JA and MeJA. However, higher concentrations of these elicitors had a negative effect on the growth of the shoot cultures, while simultaneously resulting in an increase in the secondary metabolite content. The elicitor MeJA (especially 200 ?M MeJA) was more effective in terms of increasing the phenolic compound contents. The highest amounts of rutin (2.8 fold), astragalin (2.4 fold), protocatechuic acid (2.4 fold), hesperidin (2 fold), pseudohypericin (1.9 fold), chlorogenic acid (1.4 fold), and hypericin (1.9 fold) were obtained from the shoots elicited with MeJA (200 ?M). In addition, the application of elicitor JA 200 ?M increased the amount of luteolin (2 fold), quercitrin (1.9 fold), apigetrin (4 fold), apigenin (2.9 fold), and hyperoside (1.3 fold). The current study revealed that specific secondary plant metabolites can be regulated by exogenous elicitors in shoot cultures of H. ambysepalum, thus highlighting their promise as a very valuable source of raw materials for the pharmaceutical industry.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"67 1","pages":"0"},"PeriodicalIF":0.9000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Elicitors enhanced the production of bioactive compounds in shoot cultures of Hypericum amblysepalum\",\"authors\":\"Hilal Surmuş Asan\",\"doi\":\"10.2298/botserb2302271s\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In this study, we examined the effects of elicitors MeJA (Methyl jasmonate) and JA (Jasmonic acid) (50, 100, and 200 ?M) on the growth and production of 15 phenolic compounds in shoot cultures of Hypericum amblysepalum using the LC-MS/MS method. The biomass production increased on shoots elicited with 50 ?M JA and MeJA. However, higher concentrations of these elicitors had a negative effect on the growth of the shoot cultures, while simultaneously resulting in an increase in the secondary metabolite content. The elicitor MeJA (especially 200 ?M MeJA) was more effective in terms of increasing the phenolic compound contents. The highest amounts of rutin (2.8 fold), astragalin (2.4 fold), protocatechuic acid (2.4 fold), hesperidin (2 fold), pseudohypericin (1.9 fold), chlorogenic acid (1.4 fold), and hypericin (1.9 fold) were obtained from the shoots elicited with MeJA (200 ?M). In addition, the application of elicitor JA 200 ?M increased the amount of luteolin (2 fold), quercitrin (1.9 fold), apigetrin (4 fold), apigenin (2.9 fold), and hyperoside (1.3 fold). The current study revealed that specific secondary plant metabolites can be regulated by exogenous elicitors in shoot cultures of H. ambysepalum, thus highlighting their promise as a very valuable source of raw materials for the pharmaceutical industry.\",\"PeriodicalId\":9161,\"journal\":{\"name\":\"Botanica Serbica\",\"volume\":\"67 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Botanica Serbica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2298/botserb2302271s\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Botanica Serbica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2298/botserb2302271s","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Elicitors enhanced the production of bioactive compounds in shoot cultures of Hypericum amblysepalum
In this study, we examined the effects of elicitors MeJA (Methyl jasmonate) and JA (Jasmonic acid) (50, 100, and 200 ?M) on the growth and production of 15 phenolic compounds in shoot cultures of Hypericum amblysepalum using the LC-MS/MS method. The biomass production increased on shoots elicited with 50 ?M JA and MeJA. However, higher concentrations of these elicitors had a negative effect on the growth of the shoot cultures, while simultaneously resulting in an increase in the secondary metabolite content. The elicitor MeJA (especially 200 ?M MeJA) was more effective in terms of increasing the phenolic compound contents. The highest amounts of rutin (2.8 fold), astragalin (2.4 fold), protocatechuic acid (2.4 fold), hesperidin (2 fold), pseudohypericin (1.9 fold), chlorogenic acid (1.4 fold), and hypericin (1.9 fold) were obtained from the shoots elicited with MeJA (200 ?M). In addition, the application of elicitor JA 200 ?M increased the amount of luteolin (2 fold), quercitrin (1.9 fold), apigetrin (4 fold), apigenin (2.9 fold), and hyperoside (1.3 fold). The current study revealed that specific secondary plant metabolites can be regulated by exogenous elicitors in shoot cultures of H. ambysepalum, thus highlighting their promise as a very valuable source of raw materials for the pharmaceutical industry.
Botanica SerbicaAgricultural and Biological Sciences-Plant Science
CiteScore
1.40
自引率
12.50%
发文量
17
审稿时长
34 weeks
期刊介绍:
Botanica Serbica publishes original research papers on all aspects of plant, fungal and microbial biology research including the disciplines of microbiology, mycology, lichenology, bryology, flora, vegetation, biogeography, systematics, taxonomy, plant biotechnology, plant cell biology, plant ecology, environmental plant biology, forestry, genomics, horticulture, limnology, metabolomics, molecular biology, proteomics, virology, plant conservation and protection, and wildlife and ecosystem management.