{"title":"含木果苷的木果叶对慢性关节炎大鼠生物氧化还原失衡和贫血的缓解作用","authors":"Ashish Singhai, Yusra Ahmad, Umesh Kumar Patil","doi":"10.5530/ijper.57.4.131","DOIUrl":null,"url":null,"abstract":"Abstract: Background: Rheumatoid Arthritis (RA) is a chronic disorder affecting musculoskeletal and autoimmune system with inflammatory characteristics. Anemia is most prevailing, among other extra-articular complications in RA. These complications are analogous to exorbitant inflammation and biological redox imbalance which ultimately affects the quality life and life expectancy of RA patients. Objectives: The study was planned to investigate the palliative action of Woodfordia fruticosa (WF) leaf extracts and isolation from active fraction, on articular/ extra-articular complications in rats with Complete Freund’s Adjuvant (CFA) induced arthritis. Materials and Methods: The action of Petroleum ether (WFP) and Ethanolic (WFE) extracts of WF was assessed in CFA induced adult Lewis rats (180-200gm) arthritic rats. In prophylactic treatment they received WF extracts 100mg/kg p.o. for 28 successive days and therapeutic treatment from the 14th day of CFA injection. Active extract was then fractionated by solvent-solvent extraction and chromatographic techniques. The isolated compound was characterized by TLC and various spectroscopic studies. Results: WFP significantly alleviated the severity of arthritis in animals than WFE in 28 days. Remarkable reduction (p< 0.001) in various complications was observed including paw volume (85.42%), loss in body weight, erythrocyte sedimentation rate, anemia, locomotor activity, and lipid peroxidation of hepatic/ synovial tissues and increment in SOD (U/mg) activities. A compound was isolated and characterized by UV, TLC, FTIR, LCMS and NMR spectroscopic techniques. Conclusion: WFP at 100mg/kg was advantageous in the superintendence of RA intricacies which was also confirmed by the histological and radiological studies. And a tannin compound Woodfruticosin was isolated. Keywords: Rheumatoid arthritis, Chromatography, Isolation, Spectroscopy, Woodfruticosin","PeriodicalId":13407,"journal":{"name":"Indian Journal of Pharmaceutical Education and Research","volume":"29 1","pages":"0"},"PeriodicalIF":0.8000,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Palliative Action of Woodfordia fruticosa Leaves Containing Woodfruticosin on Biological Redox Imbalance and Anemia in CFA–Induced Arthritic Rats\",\"authors\":\"Ashish Singhai, Yusra Ahmad, Umesh Kumar Patil\",\"doi\":\"10.5530/ijper.57.4.131\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract: Background: Rheumatoid Arthritis (RA) is a chronic disorder affecting musculoskeletal and autoimmune system with inflammatory characteristics. Anemia is most prevailing, among other extra-articular complications in RA. These complications are analogous to exorbitant inflammation and biological redox imbalance which ultimately affects the quality life and life expectancy of RA patients. Objectives: The study was planned to investigate the palliative action of Woodfordia fruticosa (WF) leaf extracts and isolation from active fraction, on articular/ extra-articular complications in rats with Complete Freund’s Adjuvant (CFA) induced arthritis. Materials and Methods: The action of Petroleum ether (WFP) and Ethanolic (WFE) extracts of WF was assessed in CFA induced adult Lewis rats (180-200gm) arthritic rats. In prophylactic treatment they received WF extracts 100mg/kg p.o. for 28 successive days and therapeutic treatment from the 14th day of CFA injection. Active extract was then fractionated by solvent-solvent extraction and chromatographic techniques. The isolated compound was characterized by TLC and various spectroscopic studies. Results: WFP significantly alleviated the severity of arthritis in animals than WFE in 28 days. Remarkable reduction (p< 0.001) in various complications was observed including paw volume (85.42%), loss in body weight, erythrocyte sedimentation rate, anemia, locomotor activity, and lipid peroxidation of hepatic/ synovial tissues and increment in SOD (U/mg) activities. A compound was isolated and characterized by UV, TLC, FTIR, LCMS and NMR spectroscopic techniques. Conclusion: WFP at 100mg/kg was advantageous in the superintendence of RA intricacies which was also confirmed by the histological and radiological studies. And a tannin compound Woodfruticosin was isolated. Keywords: Rheumatoid arthritis, Chromatography, Isolation, Spectroscopy, Woodfruticosin\",\"PeriodicalId\":13407,\"journal\":{\"name\":\"Indian Journal of Pharmaceutical Education and Research\",\"volume\":\"29 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2023-10-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Indian Journal of Pharmaceutical Education and Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5530/ijper.57.4.131\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"EDUCATION, SCIENTIFIC DISCIPLINES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Pharmaceutical Education and Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5530/ijper.57.4.131","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"EDUCATION, SCIENTIFIC DISCIPLINES","Score":null,"Total":0}
Palliative Action of Woodfordia fruticosa Leaves Containing Woodfruticosin on Biological Redox Imbalance and Anemia in CFA–Induced Arthritic Rats
Abstract: Background: Rheumatoid Arthritis (RA) is a chronic disorder affecting musculoskeletal and autoimmune system with inflammatory characteristics. Anemia is most prevailing, among other extra-articular complications in RA. These complications are analogous to exorbitant inflammation and biological redox imbalance which ultimately affects the quality life and life expectancy of RA patients. Objectives: The study was planned to investigate the palliative action of Woodfordia fruticosa (WF) leaf extracts and isolation from active fraction, on articular/ extra-articular complications in rats with Complete Freund’s Adjuvant (CFA) induced arthritis. Materials and Methods: The action of Petroleum ether (WFP) and Ethanolic (WFE) extracts of WF was assessed in CFA induced adult Lewis rats (180-200gm) arthritic rats. In prophylactic treatment they received WF extracts 100mg/kg p.o. for 28 successive days and therapeutic treatment from the 14th day of CFA injection. Active extract was then fractionated by solvent-solvent extraction and chromatographic techniques. The isolated compound was characterized by TLC and various spectroscopic studies. Results: WFP significantly alleviated the severity of arthritis in animals than WFE in 28 days. Remarkable reduction (p< 0.001) in various complications was observed including paw volume (85.42%), loss in body weight, erythrocyte sedimentation rate, anemia, locomotor activity, and lipid peroxidation of hepatic/ synovial tissues and increment in SOD (U/mg) activities. A compound was isolated and characterized by UV, TLC, FTIR, LCMS and NMR spectroscopic techniques. Conclusion: WFP at 100mg/kg was advantageous in the superintendence of RA intricacies which was also confirmed by the histological and radiological studies. And a tannin compound Woodfruticosin was isolated. Keywords: Rheumatoid arthritis, Chromatography, Isolation, Spectroscopy, Woodfruticosin
期刊介绍：
The official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967. IJPER, a quarterly publication devoted to publish reviews and research articles in pharmacy and the related disciplines of Pharmaceutical education. It mainly covers the articles of special interest, covering the areas of Pharmaceutical research, teaching and learning, laboratory innovations, education technology, curriculum design, examination reforms, training and other related issues. It encourages debates and discussions on the issues of vital importance to Pharmaceutical education and research. The goal of the journal is to provide the quality publications and publish most important research and review articles in the field of drug development and pharmaceutical education. It is circulated and referred by more than 6000 teachers, 40,000 students and over 1000 professionals working in Pharmaceutical industries, Regulatory departments, hospitals etc.
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