{"title":"新冠肺炎暴发后肺炎支原体肺炎流行病学及遗传特征分析","authors":"Lin Li, Bing-han Wang, Wei Li","doi":"10.1055/s-0043-1776043","DOIUrl":null,"url":null,"abstract":"Abstract Objective Mycoplasma pneumoniae pneumonia (MPP) accounts for a major part of community-acquired pneumonia in children, and we performed this study to investigate the epidemiological and genetic characteristics of MPP after the outbreak of COVID-19. Methods A total of 15,538 throat swab samples were collected from inpatients with respiratory tract infections from January 2021 to December 2021. All specimens were detected by real-time reverse transcriptase polymerase chain reaction (RT-PCR). The P1 gene of Mycoplasma pneumoniae (MP) in positive samples was amplified and sequenced. Results From January 2021 to December 2021, a total of 15,538 children with acute respiratory tract infection were tested by real-time RT-PCR in our study, including 9,056 boys and 6,482 girls. Overall, 469 (3.0%, 469/15,538) tested positive for MP, with 266 (2.9%, 266/9,056) males and 203 (3.1%, 203/6,482) females (p = 0.48). The positive rates of MP infection in < 1 year old, 1 to 3 years old, 3 to 5 years old, 5 to 7 years old, and >7 years old groups were 1.31% (85/6,474), 1.87% (64/3,423), 3.65% (95/2,601), 8.02% (127/1,583), and 6.72% (98/1,458), respectively. The homology analysis of the P1 gene of 24 MP positive samples showed that the nucleotide sequence consistency was up to 91.2 to 99.3%. Conclusion After the outbreak of COVID-19, positive detection rate was the highest from 5 to 7 years old among children with MPP, and the genotype of MP in Hangzhou, China area was focused on P1 subtype, type I.","PeriodicalId":16739,"journal":{"name":"Journal of Pediatric infectious diseases","volume":"12 4","pages":"0"},"PeriodicalIF":0.2000,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Epidemiological and Genetic Characteristics of Mycoplasma pneumoniae Pneumonia after the Outbreak of COVID-19\",\"authors\":\"Lin Li, Bing-han Wang, Wei Li\",\"doi\":\"10.1055/s-0043-1776043\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract Objective Mycoplasma pneumoniae pneumonia (MPP) accounts for a major part of community-acquired pneumonia in children, and we performed this study to investigate the epidemiological and genetic characteristics of MPP after the outbreak of COVID-19. Methods A total of 15,538 throat swab samples were collected from inpatients with respiratory tract infections from January 2021 to December 2021. All specimens were detected by real-time reverse transcriptase polymerase chain reaction (RT-PCR). The P1 gene of Mycoplasma pneumoniae (MP) in positive samples was amplified and sequenced. Results From January 2021 to December 2021, a total of 15,538 children with acute respiratory tract infection were tested by real-time RT-PCR in our study, including 9,056 boys and 6,482 girls. Overall, 469 (3.0%, 469/15,538) tested positive for MP, with 266 (2.9%, 266/9,056) males and 203 (3.1%, 203/6,482) females (p = 0.48). The positive rates of MP infection in < 1 year old, 1 to 3 years old, 3 to 5 years old, 5 to 7 years old, and >7 years old groups were 1.31% (85/6,474), 1.87% (64/3,423), 3.65% (95/2,601), 8.02% (127/1,583), and 6.72% (98/1,458), respectively. The homology analysis of the P1 gene of 24 MP positive samples showed that the nucleotide sequence consistency was up to 91.2 to 99.3%. Conclusion After the outbreak of COVID-19, positive detection rate was the highest from 5 to 7 years old among children with MPP, and the genotype of MP in Hangzhou, China area was focused on P1 subtype, type I.\",\"PeriodicalId\":16739,\"journal\":{\"name\":\"Journal of Pediatric infectious diseases\",\"volume\":\"12 4\",\"pages\":\"0\"},\"PeriodicalIF\":0.2000,\"publicationDate\":\"2023-11-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Pediatric infectious diseases\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1055/s-0043-1776043\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pediatric infectious diseases","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1055/s-0043-1776043","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Epidemiological and Genetic Characteristics of Mycoplasma pneumoniae Pneumonia after the Outbreak of COVID-19
Abstract Objective Mycoplasma pneumoniae pneumonia (MPP) accounts for a major part of community-acquired pneumonia in children, and we performed this study to investigate the epidemiological and genetic characteristics of MPP after the outbreak of COVID-19. Methods A total of 15,538 throat swab samples were collected from inpatients with respiratory tract infections from January 2021 to December 2021. All specimens were detected by real-time reverse transcriptase polymerase chain reaction (RT-PCR). The P1 gene of Mycoplasma pneumoniae (MP) in positive samples was amplified and sequenced. Results From January 2021 to December 2021, a total of 15,538 children with acute respiratory tract infection were tested by real-time RT-PCR in our study, including 9,056 boys and 6,482 girls. Overall, 469 (3.0%, 469/15,538) tested positive for MP, with 266 (2.9%, 266/9,056) males and 203 (3.1%, 203/6,482) females (p = 0.48). The positive rates of MP infection in < 1 year old, 1 to 3 years old, 3 to 5 years old, 5 to 7 years old, and >7 years old groups were 1.31% (85/6,474), 1.87% (64/3,423), 3.65% (95/2,601), 8.02% (127/1,583), and 6.72% (98/1,458), respectively. The homology analysis of the P1 gene of 24 MP positive samples showed that the nucleotide sequence consistency was up to 91.2 to 99.3%. Conclusion After the outbreak of COVID-19, positive detection rate was the highest from 5 to 7 years old among children with MPP, and the genotype of MP in Hangzhou, China area was focused on P1 subtype, type I.
期刊介绍:
The Journal of Pediatric Infectious Diseases is a peer-reviewed medical journal publishing articles in the field of child infectious diseases. The journal provides an in-depth update on new subjects and current comprehensive coverage of the latest techniques used in diagnosis and treatment of childhood infectious diseases.
The following articles will be considered for publication: editorials, original and review articles, rapid communications, letters to the editor and book reviews. The aim of the journal is to share and disseminate knowledge between all disciplines in the field of pediatric infectious diseases.