Neurobehavioral and biochemical effects of choline chloride administration in rats

Background and Objective: Choline is an essential nutrient related to vitamin B complex activity.  It can be received through different food sources. It is an important component of acetylcholine which is involved in different brain functions. The present study is designed to assess the behavioral effects of choline chloride in animal models. Methods: This case-control study was performed in the Department of Biochemistry, Federal Urdu University, Karachi for eight months from May 2022 to December 2022. Twenty-four adult male albino Wister rats were divided into four groups, one control and three test groups. Choline chloride dissolved in saline was given intraperitoneally at 25mg/ml, 50mg/ml and 100mg/ml to the test groups respectively, and saline was given to the control animals for two weeks. Memory and depression-like symptoms were monitored. Morris water maze and brain acetylcholine levels were used for memory functions and force swimming test was performed to monitor depression-like symptoms. Results: The present study showed significantly improving memory functions and increase in immobility time exhibited by choline-treated rats indicating depression-like symptoms in rats. The present investigation also shows that serum level as well as glucose level increase in dose dependent manners. Increased depression-like symptoms may be attributed due to onset of diabetes or increased glucose concentration. Conclusion: In conclusion choline administration at different doses (25mg/ml, 50mg/ml, and 100mg/ml) produces behavioral and biochemical effects in rats.