A fully automated touch-response behavior inspection pipeline on zebrafish larvae

Abstract A touch-evoked response of zebrafish larvae provides information on the mechanism of the gene functional expressions. Recently, an automated system has been developed for precise and repeated touch-response experimentation with minor human intervention. To quantify the collected data, we propose a fully automated multi-larvae touch-response behavior inspection pipeline based on larva tracking and segmentation. Experimental data with different treatments is analyzed by using the proposed inspection platform for demonstration, and the result proves that this platform can generate comparable touch-response behavior inspection readouts efficiently and automatically. The initial results were published in 31. Workshop Computational Intelligence, and this paper summarizes and extends the main work of the respective article.