植物内生芽孢杆菌BTR11在防治细菌性叶枯病和促进水稻生长中的应用

IF 2.1 3区 农林科学 Q1 ENTOMOLOGY Egyptian Journal of Biological Pest Control Pub Date : 2023-09-25 DOI:10.1186/s41938-023-00740-w
Trung Quang Do, Tri Trong Nguyen, Van Mai Dinh
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引用次数: 0

摘要

背景水稻黄单胞菌(Xanthomonas oryzae pv)引起的细菌性叶枯病(BLB)。米曲菌(Xoo)是造成水稻严重产量损失的最具破坏性的病原菌之一。应用具有植物生长促进作用的细菌生物防治剂(BCA)是一种很有前途的方法,可以替代目前的常规方法来控制BLB病,提高作物产量。因此,本研究旨在从健康水稻和Xoo病水稻的内部组织和根际土壤中分离和鉴定具有PGP能力的BCA,并在温室和田间条件下评价它们的拮抗和PGP性能。结果共分离出14株对Xoo具有体外拮抗能力的细菌。水稻内生细菌velezensis (Bacillius velezensis, BTR11)对Xoo的拮抗活性最高,能产生吲哚乙酸(IAA)和矿化营养物质(K和P)。温室试验结果表明,在保护对抗模式下,即在Xoo侵染前后,菌株BTR11培养液对BLB具有较高的保护效果(72.1%)。田间试验初步结果表明,在田间病害发生时,将该菌种作为苗土添加剂与喷施相结合,对白叶枯病的防治效果可达75 ~ 85%。此外,使用BTR11菌株也使水稻产量比未处理的对照提高了约12%。结论利用内生细菌防治褐枯草病,促进植物生长,减少农药在水稻栽培中的使用,具有重要的应用潜力。
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Application of endophytic bacterium Bacillus velezensis BTR11 to control bacterial leaf blight disease and promote rice growth
Abstract Background Bacterial leaf blight (BLB) disease caused by Xanthomonas oryzae pv. oryzae ( Xoo ) is one of the most destructive pathogens responsible for severe yield losses in rice. Application of bacterial biocontrol agent (BCA) with plant growth promotion (PGP) abilities is a promising method that alternates current conventional practices to manage BLB disease and increase crop yield. Hence, this study aimed to isolate and identify BCA with PGP abilities from internal plant tissues and rhizosphere soil of healthy and Xoo -infected rice and evaluate their antagonistic and PGP properties under greenhouse and field conditions. Results A total of 14 bacterial isolates were isolated and presented in vitro antagonistic ability against the Xoo. The rice endophytic bacterium strain Bacillius velezensis ( BTR11) had the highest antagonistic activity against the Xoo , produced indole acetic acid (IAA), and mineralized nutrients (K and P). The greenhouse experiment revealed that culture broth of strain BTR11 had a high protective effect (72.1%) against the BLB when applied at the protective-fighting mode, i.e., before and after the Xoo infection. Preliminary results of the field experiment showed that a protective effect against the bacterial blight disease was obtained as high as 75–85%, if the strain was used as an additive to the soil for the seedlings in combination with spraying when the disease began in the field. In addition, using the strain BTR11 also increased the rice yield to about 12% more than the un-treatment control. Conclusions The study showed a significant potential for the application of endophytic bacteria in controlling BLB disease, while stimulating plant growth, thus reducing the use of agrochemicals in rice cultivation.
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来源期刊
CiteScore
4.90
自引率
16.70%
发文量
128
审稿时长
>12 weeks
期刊介绍: The Egyptian Journal of Biological Pest Control is a periodic scientific journal published by the Egyptian Society for Biological Control of Pests (ESBCP) in collaboration with SpringerNature. The journal aims to publish internationally peer-reviewed, high-quality research articles in the field of biological and integrated pest control (non-chemical control). The journal publishes review articles, original papers, conference reports, book reviews, editorials, laboratory reports, technical notes and short communications.
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