从酸橙皮中提取生物活性香豆素作为样品色谱前预处理

IF 0.9 Q4 CHEMISTRY, MULTIDISCIPLINARY Acta Chimica Slovaca Pub Date : 2023-01-01 DOI:10.2478/acs-2023-0007
Lucia Krasňanská, Katarína Hroboňová, Andrea Špačková
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引用次数: 0

摘要

对酸橙皮进行了分析,以评估其在各种预期用途中的适用性,例如,在香水、化妆品和清洁产品中的应用,或作为生物活性或其他增值化合物的来源。有针对性的分析可以使这些天然材料的废料部分得到更广泛的利用。本文介绍了一种高效、简便、省时的酸橙皮香豆素分析方法,包括样品提取预处理和荧光检测(FLD)高效液相色谱(HPLC)定量。超声辅助提取的最佳条件为:水为提取溶剂,温度为40℃,提取时间为10 min。采用磁性分子印迹聚合物作为固相萃取吸附剂,对香豆素进行一次萃取清洗、分离和富集,然后进行色谱分析。herniarin和bellliferone的回收率大于86% (RSD≤4.8%)。HPLC-FLD法在50 ~ 1000 ng/mL的线性范围内,相关系数大于0.998。herniarin和bellliferone的定量限分别为8.2和44 ng/mL。这些结果表明,所提出的样品前处理方法适用于分析目的,也适用于其他柑橘样品的分析,以及将来从柑橘皮中大规模制备分离生物活性香豆素。
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Extraction of bioactive coumarins from lime peel as sample pretreatment before chromatographic analysis
Abstract Analysis of lime peel was applied to assess their suitability for various intended purposes, e.g., application in perfumery, cosmetics, and cleaning products, or as a source of bioactive or other value-added compounds. Targeted analysis allows wider usability of the waste part of these natural materials. In the present study, a novel, efficient, lab-simple, time-saving analytical method for coumarins determination in lime peel, including sample pretreatment by extraction and quantification by HPLC with fluorescence detection (FLD), is introduced. Optimal conditions of ultrasound assisted extraction included water as extraction solvent, temperature of 40 °C, and extraction time of 10 min. A magnetic molecularly imprinted polymer was employed as solid phase extraction adsorbent for primary extract cleaning, isolation, and enrichment of coumarins before chromatographic analysis. Recovery of herniarin and umbelliferone was more than 86 % ((RSD ≤ 4.8 %). Linear range of 50—1000 ng/mL with correlation coefficient above 0.998 was obtained for the proposed HPLC-FLD method. The limit of quantification was 8.2 and 44 ng/mL for herniarin and umbelliferone, respectively. These results show that the proposed sample pretreatment procedure is suitable for analytical purposes and is perspective also for the analysis of other citrus samples, as well as for future scale-up preparative isolation of bioactive coumarins from citrus peel.
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来源期刊
Acta Chimica Slovaca
Acta Chimica Slovaca CHEMISTRY, MULTIDISCIPLINARY-
自引率
12.50%
发文量
11
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