金盏花总黄酮和总酚含量及其抗菌和抗氧化活性研究索尔

Falah Saleh Mohammed, Imran Uysal, Ali Erdem Sabik, Muhittin Dogan, Mustafa Sevindik
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引用次数: 0

摘要

人类发现植物有多种用途。它不仅从营养的角度对你有好处,而且还被用来治疗各种疾病。在本研究中,我们分析了金盏花银行和;因为它具有抗氧化和抗菌的特性。同时计算总酚和类黄酮浓度。为此,使用索氏装置从植物的地上部分提取乙醇和甲醇。采用Rel Assay试剂盒测定抗氧化剂的有效性。用琼脂稀释法测定了其对多种细菌和真菌的抑菌活性。用Folin-Ciocalteu试剂定量测定总酚含量。用氯化铝法测定总黄酮浓度。研究发现,该植物提取物的TAS值为4.974±0.259 mmol/L, TOS值为12.437±0.150µmol/L, OSI值为0.251±0.014。结果表明,该植物提取物在100 ~ 400µg/mL浓度范围内对细菌菌株有效,在50 ~ 100µg/mL浓度范围内对真菌菌株有效。乙醇提取物也具有较高的总酚浓度和总黄酮含量。在本研究中,显示出长曲菌具有抗菌和抗氧化的特性。
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TOTAL PHENOLIC AND FLAVONOID CONTENTS, ANTIMICROBIAL AND ANTIOXIDANT POTENTIALS OF CAMPANULA STRIGOSA BANKS & SOL
Humans find several uses for plants. Not only is it good for you from a nutritional standpoint, but it has also been used to cure a wide range of illnesses. In this research, we analysed Campanula strigosa Banks & Sol. for its antioxidant and antibacterial properties. Total phenolic and flavonoid concentrations were also calculated. For this purpose, a soxhlet apparatus was used to extract ethanol and methanol from the plant's above-ground portions. The effectiveness of antioxidants was measured using Rel Assay kits. The agar dilution technique was used to test for antimicrobial activity against various bacterial and fungus species. Folin-Ciocalteu reagent was used to quantify total phenolic content. An aluminium chloride assay determined the total flavonoid concentration. Studies found that the plant extract had a TAS value of 4.974±0.259 mmol/L, a TOS value of 12.437±0.150 µmol/L, and an OSI value of 0.251±0.014. The plant extract was shown to be effective against bacterial strains at concentrations between 100 and 400 µg/mL, and against fungal strains at concentrations between 50 and 100 µg/mL. Ethanol extract also had a greater total phenolic concentration and total flavonoid content. C. strigosa was shown to have antibacterial and antioxidant properties in this study.
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