体外研究间充质干细胞和子宫上皮细胞分泌组的影响布法罗的囊胚生产

Vikash Chandra, G. Taru Sharma
{"title":"体外研究间充质干细胞和子宫上皮细胞分泌组的影响布法罗的囊胚生产","authors":"Vikash Chandra, G. Taru Sharma","doi":"10.18311/jer/2023/34992","DOIUrl":null,"url":null,"abstract":"One challenge that needs to be addressed in animal embryo production is to create the appropriate in vitro culture to improve the blastocyst rate and produce high-quality embryos. Buffalo Mesenchymal Stem Cells (MSCs) were derived from Wharton’s jelly and expanded in vitro. Conditioned media (secretome) was collected from well-characterized WJMSCs at 3rd passage. Similarly, buffalo Uterine Epithelial Cells (UECs) were derived from nongravid uteri and expanded in vitro. The secretome was collected from a well-characterized first passage UECs monolayer primed with steroid hormones (progesterone 3.14ng/ml and estradiol-17β 5 31pg/ml). Culture media was replaced with non-serum media, and the media was collected after 72h. Day 4 IVF-derived embryos were cultured in three groups: in regular mSOF media (Group I), mSOF replaced with 50% CM derived from MSCs (Group II), and mSOF replaced with 50% CM from steroid-treated UECs (Group III). Blastocyst rates were evaluated on day 09 post IVF. The blastocyst rate in group II was significantly higher (p < 0.05) than the control group, which was further enhanced in group III. In vitro co-culture of embryos with the secretome derived from mesenchymal stem cells or steroid-treated UECs improved the blastocyst rate. UECs and their secretions are essential to establish uterine receptivity and to mimic the internal in vivo environment.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"26 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Elucidating the Impact of Secretome Derived from Mesenchymal Stem Cell and Uterine Epithelial Cells During &lt;i&gt;In Vitro&lt;/i&gt; Blastocyst Production in Buffalo\",\"authors\":\"Vikash Chandra, G. Taru Sharma\",\"doi\":\"10.18311/jer/2023/34992\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"One challenge that needs to be addressed in animal embryo production is to create the appropriate in vitro culture to improve the blastocyst rate and produce high-quality embryos. Buffalo Mesenchymal Stem Cells (MSCs) were derived from Wharton’s jelly and expanded in vitro. Conditioned media (secretome) was collected from well-characterized WJMSCs at 3rd passage. Similarly, buffalo Uterine Epithelial Cells (UECs) were derived from nongravid uteri and expanded in vitro. The secretome was collected from a well-characterized first passage UECs monolayer primed with steroid hormones (progesterone 3.14ng/ml and estradiol-17β 5 31pg/ml). Culture media was replaced with non-serum media, and the media was collected after 72h. Day 4 IVF-derived embryos were cultured in three groups: in regular mSOF media (Group I), mSOF replaced with 50% CM derived from MSCs (Group II), and mSOF replaced with 50% CM from steroid-treated UECs (Group III). Blastocyst rates were evaluated on day 09 post IVF. The blastocyst rate in group II was significantly higher (p < 0.05) than the control group, which was further enhanced in group III. In vitro co-culture of embryos with the secretome derived from mesenchymal stem cells or steroid-treated UECs improved the blastocyst rate. UECs and their secretions are essential to establish uterine receptivity and to mimic the internal in vivo environment.\",\"PeriodicalId\":15664,\"journal\":{\"name\":\"Journal of Endocrinology and Reproduction\",\"volume\":\"26 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-09-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Endocrinology and Reproduction\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18311/jer/2023/34992\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Endocrinology and Reproduction","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18311/jer/2023/34992","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

在动物胚胎生产中需要解决的一个挑战是创造合适的体外培养,以提高囊胚率,产生高质量的胚胎。布法罗间充质干细胞(MSCs)来源于沃顿水母并在体外扩增。条件培养基(分泌组)在第三代时从具有良好特征的WJMSCs中收集。同样,水牛子宫上皮细胞(UECs)也来源于非妊娠子宫并在体外扩增。分泌组收集自具有良好特征的首代UECs单层,并注入类固醇激素(孕酮3.14ng/ml和雌二醇-17β 5 31pg/ml)。用无血清培养基代替培养基,72h后收集培养基。第4天的体外受精胚胎分为三组:在常规的mSOF培养基中培养(第一组),用MSCs来源的50% CM代替mSOF(第二组),用类固醇处理的uec来源的50% CM代替mSOF(第三组)。在体外受精后第09天评估囊胚率。II组的囊胚率显著高于对照组(p <0.05),且在ⅲ组进一步增强。胚胎与间充质干细胞分泌组或类固醇处理的uec体外共培养可提高囊胚率。uec及其分泌物是建立子宫接受性和模拟体内环境所必需的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Elucidating the Impact of Secretome Derived from Mesenchymal Stem Cell and Uterine Epithelial Cells During <i>In Vitro</i> Blastocyst Production in Buffalo
One challenge that needs to be addressed in animal embryo production is to create the appropriate in vitro culture to improve the blastocyst rate and produce high-quality embryos. Buffalo Mesenchymal Stem Cells (MSCs) were derived from Wharton’s jelly and expanded in vitro. Conditioned media (secretome) was collected from well-characterized WJMSCs at 3rd passage. Similarly, buffalo Uterine Epithelial Cells (UECs) were derived from nongravid uteri and expanded in vitro. The secretome was collected from a well-characterized first passage UECs monolayer primed with steroid hormones (progesterone 3.14ng/ml and estradiol-17β 5 31pg/ml). Culture media was replaced with non-serum media, and the media was collected after 72h. Day 4 IVF-derived embryos were cultured in three groups: in regular mSOF media (Group I), mSOF replaced with 50% CM derived from MSCs (Group II), and mSOF replaced with 50% CM from steroid-treated UECs (Group III). Blastocyst rates were evaluated on day 09 post IVF. The blastocyst rate in group II was significantly higher (p < 0.05) than the control group, which was further enhanced in group III. In vitro co-culture of embryos with the secretome derived from mesenchymal stem cells or steroid-treated UECs improved the blastocyst rate. UECs and their secretions are essential to establish uterine receptivity and to mimic the internal in vivo environment.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Impacts of Protein-, L-Tryptophan-, Carbohydrate-, Oil-Rich Diets on Growth Performance, Levels of Melatonin, Oxidative Stress, Antioxidative Agents, and Vital Digestive Enzymes in the Gut of Juvenile Carp (Catla catla) Adipose Tissue Dysfunction in PCOS An Update on the Genetics of Polycystic Ovary Syndrome Evaluating the Relative Efficacy of Synthetic and Natural Drugs in Endometriosis Adopting Molecular Modelling Approach Elucidating the Impact of Secretome Derived from Mesenchymal Stem Cell and Uterine Epithelial Cells During <i>In Vitro</i> Blastocyst Production in Buffalo
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1