黄颡鱼(Pelteobagrus fulvidraco)一种智力素基因的分子鉴定与表达分析

IF 2.1 3区 农林科学 Q2 FISHERIES Fishes Pub Date : 2023-10-02 DOI:10.3390/fishes8100492
Senhao Jiang, Yuting Lei, Yanxuan Li, Wanyan Sun, Ti Wang, Ruiting Ma, Qiuning Liu, Boping Tang
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引用次数: 0

摘要

智力素(ITLNs)是一类钙依赖性凝集素,具有碳水化合物结合能力,分布在各种脊椎动物中,在抵抗病原体感染的先天免疫反应中发挥重要作用。黄颡鱼(黄颡目:黄颡科)是中国重要的经济鱼类。本研究的目的是量化ITLN在病原体相关分子模式(pathogen-associated molecular patterns, PAMPs)刺激下的基因表达。本文对P. fulvidraco的ITLN基因进行了表征,命名为PfITLN。PfITLN全长1132 bp,包括一个140 bp的5 ' -未翻译区(UTR)、一个110 bp的3 ' -未翻译区(UTR)和一个882 bp的开放阅读框(ORF),编码293个氨基酸的多肽,该多肽包含一个信号肽和两个纤维蛋白原相关结构域(FReDs)。PfITLN分子量为32.39 kDa,理论pI为5.03。推导出的PfITLN氨基酸序列与伊塔鲁(Ictalurus furcatus)、达尼奥(Danio)和智人(Homo sapiens)的同源性分别为81%、64%和55%。此外,预测的PfITLN三级蛋白结构与其他动物高度相似,系统发育分析表明,PfITLN蛋白与其他Teleostei接近。实时定量逆转录- pcr (qRT-PCR)分析显示,PfITLN在所有检测组织中均有表达,肝脏中丰度最高,其次是头肾、脾、干肾和肌肉。脂多糖(LPS)和多核糖素多核糖素酸(poly I:C)感染PAMP后,PfITLN在不同时间点的表达水平均显著上调。这些结果提示PfITLN可能参与先天免疫。
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Molecular Identification and Expression Analysis of an Intelectin Gene in the Yellow Catfish Pelteobagrus fulvidraco (Siluriformes: Bagridae)
Intelectins (ITLNs) are a family of calcium-dependent lectins with carbohydrate-binding capacity, are distributed across various vertebrates, and play an important role in the innate immune response against pathogen infection. The yellow catfish Pelteobagrus fulvidraco (Siluriformes: Bagridae) is an economically important fish in China. The aim of this study was to quantify the gene expression of ITLN in response to pathogen-associated molecular patterns (PAMPs) stimulation. Here, the ITLN gene of P. fulvidraco was characterized and named PfITLN. The full-length cDNA of PfITLN was 1132 bp, including a 5’-untranslated region (UTR) of 140 bp, a 3’-UTR of 110 bp, and an open reading frame (ORF) of 882 bp encoding a polypeptide of 293 amino acids, which contains a signal peptide and two fibrinogen-related domains (FReDs). PfITLN had a molecular weight of 32.39 kDa with a theoretical pI of 5.03. The deduced PfITLN amino acid sequence had 81%, 64%, and 55% homology with Ictalurus furcatus, Danio rerio, and Homo sapiens, respectively. Moreover, the predicted tertiary protein structure of PfITLN was highly similar to that of other animals, and phylogenetic analysis showed that the PfITLN protein was close to those of other Teleostei. Real-time quantitative reverse transcription-PCR (qRT-PCR) analysis showed PfITLN expression in all examined tissues, with the highest abundance seen in the liver, followed by the head kidney, spleen, trunk kidney, and muscle. After PAMP infection with lipopolysaccharide (LPS) and polyriboinosinic polyribocytidylic acid (poly I:C), the expression levels of PfITLN were significantly upregulated at different time points. These results suggested that PfITLN might be involved in innate immunity.
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Fishes
Fishes Multiple-
CiteScore
1.90
自引率
8.70%
发文量
311
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