Shuang Gang, Shirui Zhang, Shunyan Zhang, Jia Sun, Caixia Yang
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引用次数: 0
摘要
本研究对Commelina communis L.叶片样本的转录序列和RT-PCR检测进行了研究。选取病叶样本进行转录组测序，得到两个长度分别为5845和3450核苷酸(nt)的contigs。用7对引物进行RT-PCR鉴定，确定RNA1和RNA2 (GenBank MZ571836和MZ571837)分别为5880 nt和3478 nt。同源性分析和进化关系分析显示，与BBWV2 (MN786954和MN786955)在nt和氨基酸水平上的同源性最高，分别为97.5%和88.4%。这是世界上首次报道猪乙型肝炎病毒感染的病例。利用特异性引物R2-2F/R鉴定了BBWV2与c.s communis病的关联，阳性片段仅在病株中获得，提示BBWV2与该病存在关联。中国生物医学工程学报，52(2):569-574,2023(6):特刊
Broad bean wilt virus 2 in commelina communis L. in China
In the present study transcriptone sequencing and RT-PCR detection of leaf sample of Commelina communis L. were investigated. A diseased leaf sample was selected for transcriptome sequencing and two contigs with lengths of 5845 and 3450 nucleotides (nt) were obtained. They were confirmed by RT-PCR with seven pairs of primers, and RNA1 and RNA2 (GenBank MZ571836 and MZ571837) were determined to be 5880 nt and 3478 nt, respectively. Homology analysis and evolutionary relationship analysis showed the highest homology with BBWV2 (MN786954 and MN786955), for 97.5 and 88.4% at the nt and amino acid (aa) levels, respectively. This is the first report of BBWV2 infection in C. communis in the world. Specific primers R2-2F/R were used to assess the association of BBWV2 with C. communis disease, and positive fragments were obtained only in the diseased plants, indicating the association of BBWV2 with the disease. Bangladesh J. Bot. 52(2): 569-574, 2023 (June) Special
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