组织蛋白酶K和酒石酸盐抗性酸性磷酸酶5b在南印度人群不同阶段类风湿关节炎患者中的相关性

Sharmil Thangavel, Ezhilarasi Krishnamoorthy, Shyam Sundar Jaganathan, Abirami M. Padmanaban, Shila Samuel
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摘要

该研究的目的是利用28个关节的疾病活动度评分(DAS28)来确定来自南印度人群的不同阶段的类风湿性关节炎患者骨密度(BMD)变化的有效生物标志物,DAS28被用作评估类风湿性关节炎患者疾病活动度的测量方法。这项研究是在92名风湿性关节炎患者中进行的。ELISA法检测血清抗酒石酸磷酸酶-5b (TRAP-5b)和组织蛋白酶K水平。由风湿病主治医师记录血清类风湿因子(RF)和c反应蛋白(CRP)。用乳胶免疫比浊法定量c反应蛋白(CRP)。用免疫比浊法测定类风湿关节炎(RA)。骨密度(BMD) t评分根据WHO指南计算。采用反转录PCR (RT-PCR)检测组织蛋白酶k的表达。类风湿关节炎(RA)患者根据DAS28评分分为非活性组(DAS≤3.2)、中度活性组(DAS >3.2≤5.1)和非常活跃(DAS >5.1)在入学时。92例患者中,16例(17.4%)患者病情不活跃,42例(45.6%)患者病情中度活跃,34例(37%)患者病情非常活跃。非常活跃组平均骨密度显著降低(0.28±0.04;p & lt;0.001),而中度活动组(0.71±0.13;p & lt;0.001)和无活性(1.21±0.14)。非常活跃组TRAP5b和组织蛋白酶K显著升高(p <0.001),与中度运动组相比(p <0.001)和不活动组。综上所述,本研究发现的生物标志物TRAP-5b和组织蛋白酶K可能成为类风湿关节炎新的高特异性生物标志物
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Association of Cathepsin K and Tartrate- Resistant Acid Phosphatase-5b in Different Stages of Rheumatoid Arthritis Patients in South Indian Population
The aim of the study was to identify effective biomarkers of changes in bone mineral density (BMD) at different stages of rheumatoid arthritis patients from South Indian population, using disease activity score in 28 joints (DAS28) which is being used as a measurement for assessing disease activity in patients with rheumatoid arthritis. The study was carried out in 92 rheumatoid arthritis patients. Serum level of tartrateresistant acid phosphatase-5b (TRAP-5b) and cathepsin K was measured using ELISA. Serum rheumatoid factor (RF) and C-reactive protein (CRP) was recorded by the attending rheumatologists. The C-reactive protein (CRP) was quantified using a latex immunoturbidimetric method. The rheumatoid arthritis (RA) was measured by turbidimetric immunoassay method. The bone mineral density (BMD) T-score was calculated according to WHO guidelines. Reverse transcription PCR (RT-PCR) was used to determine the expression of cathepsin K. The rheumatoid arthritis (RA) patients were categorized into three groups based on the DAS28 score as inactive (DAS ≤ 3.2), moderately active (DAS > 3.2 ≤ 5.1) and very active (DAS > 5.1) at the time of admission. Out of 92 patients, 16 (17.4%) patients had inactive disease condition, 42 (45.6%) patients had moderately active disease condition and 34 (37%) patients had very active disease condition. The mean BMD was significantly lower in very active (0.28 ± 0.04; p < 0.001) as compared with moderately active (0.71 ± 0.13; p < 0.001) and inactive (1.21 ± 0.14). TRAP5b and cathepsin K showed significant increases in very active group (p < 0.001) as compared with moderately active (p < 0.001) and inactive groups. In conclusion, The biomarker TRAP-5b and cathepsin K identified in this study may become a new and highly specific biomarker for rheumatoid arthritis
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