细菌脂多糖影响、实验性代谢综合征及其联合作用下大鼠股二头肌一氧化氮循环活性

Q4 Biochemistry, Genetics and Molecular Biology Ukrainian Biochemical Journal Pub Date : 2023-09-11 DOI:10.15407/ubj95.04.024
O. Ye. Akimov, A. O. Mykytenko, V. O. Kostenko
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引用次数: 1

摘要

有证据表明,细菌脂多糖(LPS)长期刺激机体,促进促炎细胞因子和一氧化氮的分泌,可能在代谢综合征(MetS)的发展中发挥重要作用。在MetS条件下NO产生的变化有不同的方向,并依赖于特定的器官。本研究旨在研究脂多糖刺激、代谢综合征及其联合作用下大鼠股二头肌一氧化氮及其代谢产物的产生。24只雄性Wistar大鼠分为对照组、MetS组、LPS组和LPS+MetS组,为期60 d。通过在食物中加入20%的果糖溶液来复制MetS,通过腹腔注射斑疹伤寒链球菌LPS来刺激LPS。实验结果表明,在实验性代谢综合征条件下,LPS刺激大鼠机体通过l -精氨酸依赖途径增加一氧化氮的产生,但限制了代谢综合征诱导的l -精氨酸不依赖途径增加一氧化氮的产生,降低了s -亚硝基硫醇的浓度,同时增加了大鼠股二头肌中过氧亚硝酸盐和亚硝酸盐的浓度。关键词:细菌脂多糖,股二头肌,代谢综合征,一氧化氮,活性氮种
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Nitric oxide cycle activity in rat biceps femoris muscle under conditions of bacterial lipopolysaccharide influence, experimental metabolic syndrome and their combination
There is evidence that long-term organism stimulation with bacterial lipopolysaccharides (LPS), which promotes the secretion of pro-inflammatory cytokines and nitric oxide, may play an important role in metabolic syndrome (MetS) development. Changes in NO production under conditions of MetS have different directions and depend on a specific organ. The purpose of this work was to study the production of nitric oxide and its metabolites in the biceps femoris muscle of rats under conditions of lipopolysaccharide stimulation of the organism, metabolic syndrome and their combination. The study was conducted for 60 days on 24 male Wistar rats divided into control, MetS, LPS and LPS+MetS groups. MetS was reproduced by adding 20% fructose solution to food, LPS stimulation was carried out by intraperitoneal injection of S. typhi LPS. It was demonstrated that stimulation of the rat organism with LPS under conditions of experimental metabolic syndrome increased the production of nitric oxide by L-arginine-dependent pathway, but limited metabolic syndrome-induced increase in nitric oxide production by L-arginine-independent pathway, reduced the concentration of S-nitrosothiols, while increasing the concentration of peroxynitrites and nitrites in the biceps femoris muscle of rats. Keywords: bacterial lipopolysaccharide, biceps femoris, metabolic syndrome, nitric oxide, reactive nitrogen species
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来源期刊
Ukrainian Biochemical Journal
Ukrainian Biochemical Journal Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
1.20
自引率
0.00%
发文量
37
审稿时长
16 weeks
期刊介绍: The Ukrainian Biochemical Journal publishes original research papers, reviews and brief notes; papers on research methods and techniques; articles on the history of biochemistry, its development and prominent figures; discussion articles; book reviews; chronicles; etc. The journal scope includes not only biochemistry but also related sciences, such as cellular and molecular biology, bioorganic chemistry, biophysics, pharmacology, genetics, and medicine (medical biochemistry et al.) – insofar as the studies use biochemical methods and discuss biochemical findings.
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