Tillmann Lueders, Christopher Mechela, Felix Beulig, Martin Obst
{"title":"法兰克尼亚上一历史铜矿中锰氧化微生物生物膜的研究","authors":"Tillmann Lueders, Christopher Mechela, Felix Beulig, Martin Obst","doi":"10.3897/aca.6.e108107","DOIUrl":null,"url":null,"abstract":"Providing evidence for presumed chemolithoautotrophic manganese oxidation remains a major and challenging objective in subsurface microbiology. Here, we report on the dissection of blackish, leathery microbial biofilms discovered in the “Goldene Falk”, a historic copper mine in Northern Bavaria, with mine shafts originating back to the 15 th centrury. Biogeochemical analysis of the biofilm indicated a notable enrichment of manganese oxides (MnOX), with Mn making up for more than 10% (dry weight) of the deposits. STXM analysis suggested a clear biogenic origin of MnOX in situ . Characteristic nodules of MnOX with microbial cells attached were also found in aerobic Mn-oxidizing enrichment cultures set up in minimal media in the lab. The biofilms obtained from the mine were also subjected to amplicon and metagenomic sequencing,revealing a vast diversity of presumably chemolithoautotrophic and heterotrophic microbial lineages, including members of the Pyrinomonadaceae , Rhizobiales , Methylomirabilaceae and also lineages within the Nitrospiraceae previously reported to be associated with lithotrophic Mn oxidation. We reconstructed >100 high-quality bacterial genomes (MAGs), many of them carrying genomic signatures of biogenic Mn oxidation (albeit non-lithotrophic). We continue to investigate the biofilms, our enrichment cultures and the metagenomic data obtained from the mine for further evidence of possible autotrophic manganese oxidation, the macroscopic leathery biofilm representing a likely habitat for these still enigmatic microbes. Indications for nitrogen and sulfur cycling also ongoing in the biofilms will also be discussed. This research contributes to a better understanding of the yet-enigmatic capacities of the microbiota in man-made subsurface environments.","PeriodicalId":101714,"journal":{"name":"ARPHA Conference Abstracts","volume":"44 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Investigating Manganese-oxidizing microbial Biofilms in a historic Copper Mine of Upper Frankonia\",\"authors\":\"Tillmann Lueders, Christopher Mechela, Felix Beulig, Martin Obst\",\"doi\":\"10.3897/aca.6.e108107\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Providing evidence for presumed chemolithoautotrophic manganese oxidation remains a major and challenging objective in subsurface microbiology. Here, we report on the dissection of blackish, leathery microbial biofilms discovered in the “Goldene Falk”, a historic copper mine in Northern Bavaria, with mine shafts originating back to the 15 th centrury. Biogeochemical analysis of the biofilm indicated a notable enrichment of manganese oxides (MnOX), with Mn making up for more than 10% (dry weight) of the deposits. STXM analysis suggested a clear biogenic origin of MnOX in situ . Characteristic nodules of MnOX with microbial cells attached were also found in aerobic Mn-oxidizing enrichment cultures set up in minimal media in the lab. The biofilms obtained from the mine were also subjected to amplicon and metagenomic sequencing,revealing a vast diversity of presumably chemolithoautotrophic and heterotrophic microbial lineages, including members of the Pyrinomonadaceae , Rhizobiales , Methylomirabilaceae and also lineages within the Nitrospiraceae previously reported to be associated with lithotrophic Mn oxidation. We reconstructed >100 high-quality bacterial genomes (MAGs), many of them carrying genomic signatures of biogenic Mn oxidation (albeit non-lithotrophic). We continue to investigate the biofilms, our enrichment cultures and the metagenomic data obtained from the mine for further evidence of possible autotrophic manganese oxidation, the macroscopic leathery biofilm representing a likely habitat for these still enigmatic microbes. Indications for nitrogen and sulfur cycling also ongoing in the biofilms will also be discussed. This research contributes to a better understanding of the yet-enigmatic capacities of the microbiota in man-made subsurface environments.\",\"PeriodicalId\":101714,\"journal\":{\"name\":\"ARPHA Conference Abstracts\",\"volume\":\"44 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-10-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ARPHA Conference Abstracts\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3897/aca.6.e108107\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ARPHA Conference Abstracts","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3897/aca.6.e108107","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Investigating Manganese-oxidizing microbial Biofilms in a historic Copper Mine of Upper Frankonia
Providing evidence for presumed chemolithoautotrophic manganese oxidation remains a major and challenging objective in subsurface microbiology. Here, we report on the dissection of blackish, leathery microbial biofilms discovered in the “Goldene Falk”, a historic copper mine in Northern Bavaria, with mine shafts originating back to the 15 th centrury. Biogeochemical analysis of the biofilm indicated a notable enrichment of manganese oxides (MnOX), with Mn making up for more than 10% (dry weight) of the deposits. STXM analysis suggested a clear biogenic origin of MnOX in situ . Characteristic nodules of MnOX with microbial cells attached were also found in aerobic Mn-oxidizing enrichment cultures set up in minimal media in the lab. The biofilms obtained from the mine were also subjected to amplicon and metagenomic sequencing,revealing a vast diversity of presumably chemolithoautotrophic and heterotrophic microbial lineages, including members of the Pyrinomonadaceae , Rhizobiales , Methylomirabilaceae and also lineages within the Nitrospiraceae previously reported to be associated with lithotrophic Mn oxidation. We reconstructed >100 high-quality bacterial genomes (MAGs), many of them carrying genomic signatures of biogenic Mn oxidation (albeit non-lithotrophic). We continue to investigate the biofilms, our enrichment cultures and the metagenomic data obtained from the mine for further evidence of possible autotrophic manganese oxidation, the macroscopic leathery biofilm representing a likely habitat for these still enigmatic microbes. Indications for nitrogen and sulfur cycling also ongoing in the biofilms will also be discussed. This research contributes to a better understanding of the yet-enigmatic capacities of the microbiota in man-made subsurface environments.