In vitro Antimollicute Properties of Rosmarinus officinalis Extract

Rosmarinus officinalis is a widely studied plant, although its activity against bacteria of the Mollicutes class is lacking. We evaluated the antibacterial potential R. officinalis extracts and fractions against different Mycoplasma species. The most active fractions were the hexane and dichloromethane ones, with MIC of 15.62 µg mL -1 against M. capricolum and the hexane fraction with MIC of 15.62 µg mL -1 against M. pneumoniae . Subfractions were obtained by silica column chromatography, with MIC of 31.25 µg mL -1 against M. capricolum and 62.5 µg mL -1 against M. pneumoniae . Purified subfractions or the isolated compound betulinic acid did not show better activity.